RANTES和STAT3在难治性支原体肺炎患儿外周血中的表达及意义

Expression and Significance of RANTES and STAT 3 in Peripheral Blood of Children with Refractory Mycoplasma Pneumonia

目的探究受激活调节正常T细胞表达和分泌因子(RANTES)、信号转导子和转录激活子3(STAT3)在儿童支原体肺炎外周血中的表达,并评估其对难治性支原体肺炎(RMPP)的预测价值。方法选取2023年8—10月在河南省儿童医院郑州儿童医院住院的儿童支原体肺炎患儿103例,患儿均接受全程治疗,根据患儿治疗1周后是否仍出现持续发热、病情加重情况将患儿分为MPP组和RMPP组,对两组患儿外周血中RANTES、STAT3表达情况进行检测,并分析两组患儿相关实验室指标情况。收集两组患儿的一般资料,对可能诱发RMPP的因素进行单因素和多因素分析。结果RMPP组RANTES水平及STAT3 mRNA相对表达水平均高于MPP组(P<0.05)。RMPP组中性粒细胞计数、白细胞计数、C反应蛋白、血小板计数、乳酸脱氢酶以及红细胞沉降率等实验室指标均高于MPP组(P<0.05)。经单因素及多因素分析,RANTES和STAT3水平、乳酸脱氢酶水平和抗生素使用均与RMPP的发生有关(P<0.05)。结论RANTES和STAT3表达水平升高可以提示儿童支原体肺炎预后不良。

Objective To explore the expression of activated regulated normal T cell expression and secretion factors(RANTES),signal transducers and transcriptional activator 3(STAT3)in the peripheral blood of pediatric Mycoplasma pneumonia and evaluate its predictive value for refractory Mycoplasma pneumoniae pneumonia(RMPP).Methods A total of 103 children with RMPP admitted to Henan Children’s Hospital Zhengzhou Children’s Hospital from August to October 2023 were selected,all of whom received full treatment.The children were divided into general group(MPP group)and RMPP group according to whether they still had persistent fever and aggravation one week after treatment.The expression of RANTES and STAT3 in the two groups were analyzed,and the relevant laboratory indicators of the two groups were analyzed.The general data of the two groups of children were collected,the factors that may induce RMPP were analyzed by univariate and multivariate.Results The levels of RANTES and relative expression of STAT3 mRNA in the RMPP group were higher than those in the MPP group(P<0.05).The laboratory indicators such as neutrophil count,white blood cell count,C-reactive protein,platelet count,lactate dehydrogenase,and erythrocyte sedimentation rate in the RMPP group were higher than those in the MPP group(P<0.05).Through univariate and multivariate analysis,it was found that levels of RANTES and STAT3,lactate dehydrogenase,and antibiotic use were all associated with the occurrence of RMPP(P<0.05).Conclusion Elevated expression levels of RANTES and STAT3 suggest a poor prognosis of Mycoplasma pneumonia in children.