外周血炎性因子IL-10、IL-32与帕金森病认知功能障碍及非运动症状的相关性

Correlation between peripheral inflammatory cytokines IL-10 and IL-32 and cognitive dysfunction and non-motor symptoms of Parkinson's disease

目的对帕金森病患者与健康对照者进行外周血IL-10、IL-32水平测定以及认知功能及非运动症状的评估,分析IL-10、IL-32水平与帕金森病认知功能障碍及非运动症状之间的相关性。方法选取2022年1月—2023年10月于蚌埠医科大学第一附属医院就诊的帕金森病患者60例作为PD组,健康体检者60例作为对照组。采用ELISA法检测2组受试者血清IL-10、IL-32浓度水平;使用简易智力状态检查表和运动症状评价量表评估2组研究对象的认知功能及非运动症状;使用秩相关法分析外周血炎性因子IL-10、IL-32与帕金森病认知功能障碍及非运动症状的相关性。结果PD组患者外周血中IL-10水平[136.60(117.05,169.70)pg/mL]高于对照组[110.90(92.15,129.50)pg/mL,P<0.01],PD组IL-32水平[32.35(28.83,37.68)pg/mL]高于对照组[21.80(20.60,23.35)pg/mL,P<0.01];PD患者外周血IL-10水平与MMSE[(20.68±6.33)分]评分及NMSS[(103.17±11.25)分]评分均呈正相关(P<0.01),PD患者外周血IL-32水平与MMSE评分及NMSS评分均呈正相关(P<0.01)。结论PD患者外周血中炎性因子IL-10、IL-32水平较健康对照者高,并且与帕金森病认知功能障碍及非运动症状有密切的相关性。

Objective To analyze the correlation between the levels of IL-10 and IL-32 and the cognitive dysfunction and non-motor symptoms of Parkinson's disease(PD),we measured levels of IL-10 and IL-32 in the peripheral blood of Parkinson's disease patients and healthy controls and evaluated their cognitive function and non-motor symptoms.Methods Sixty patients with Parkinson's disease in the First Affiliated Hospital of Bengbu Medical University from January 2022 to October 2023 were selected as the observation group,and 60 healthy subjects were selected as the control group.Serum concentrations of IL-10 and IL-32 in the two groups were detected by ELISA.The cognitive function and non-motor symptoms of the two groups were evaluated using the simple mental state checklist and the motor symptom assessment scale.The correlation between peripheral inflammatory factors IL-10 and IL-32 and cognitive dysfunction and non-motor symptoms of Parkinson's disease was analyzed by hierarchical correlation method.Results The level of IL-10 in the peripheral blood of PD group was[136.60(117.05,169.70)pg/mL],which was higher than that of the control group[110.90(92.15,129.50)pg/mL,P<0.01].The level of IL-32 in the PD group was[32.35(28.83,37.68)pg/mL],which was higher than that in the control group[21.80(20.60,23.35)pg/mL,P<0.01].The level of IL-10 in the peripheral blood of PD patients was positively correlated with MMSE score(20.68±6.33)and NMSS score(103.17±11.25,P<0.01).The level of IL-32 in the peripheral blood in PD patients was also positively correlated with MMSE score and NMSS score(P<0.01).Conclusion The levels of inflammatory factors IL-10 and IL-32 in the peripheral blood of PD patients were higher than those of healthy controls,and were closely correlated with cognitive dysfunction and non-motor symptoms of PD.