痰热清联用抗菌药物对耐碳青霉烯铜绿假单胞菌体外抑菌的研究

Study on the in vitro antibacterial activity of Tanreqing combined with antibiotics against carbapenem resistant Pseudomonas aeruginosa

目的:研究痰热清联用抗菌药物对耐碳青霉烯铜绿假单胞菌的体外抑菌作用。方法:临床标本中分离、收集46株耐碳青霉烯铜绿假单胞菌,使用微量肉汤稀释药敏实验测出痰热清、头孢他啶、头孢哌酮舒巴坦钠各自的最低抑菌浓度(MIC值),使用微量棋盘稀释药敏实验测出痰热清联用头孢他啶、头孢哌酮钠舒巴坦钠的抑菌效果,根据计算出的FIC数值判定联合用药后的抗菌作用。结果:痰热清联合头孢他啶表现为61.4%相加作用、25.7%无关作用,联合头孢哌酮钠舒巴坦钠表现为55.7%相加作用、35.7%无关作用。结论:痰热清联用头孢他啶、头孢哌酮钠舒巴坦钠具有较好的抑菌作用。

Objective:To study the in vitro antibacterial effect of Tanreqing combined with antibiotics on carbapenem resistant Pseudomonas aeruginosa.Method:46 strains of carbapenem resistant Pseudomonas aeruginosa were isolated and collected from clinical specimens.The minimum inhibitory concentrations(MIC)of Tanreqing,Ceftadine,and Cefoperazone Sulbactam Sodium were measured using a micro broth dilution drug sensitivity test.The antibacterial effect of Tanreqing combined with Cefoperazone and Cefoperazone Sulbactam Sodium was measured using a micro chessboard dilution drug sensitivity test.The antibacterial effect of the combined medication was determined based on the calculated FIC value.Result:The combination of Tanreqing and Cefotaxime showed an additive effect of 61.4%and an unrelated effect of 25.7%,while the combination of Cefoperazone Sodium and Sulbactam Sodium showed an additive effect of 55.7%and an unrelated effect of 35.7%.Conclusion:The combination of Tan Re Qing and Cefotaxime,Cefoperazone Sodium and Sulbactam Sodium has good antibacterial effects.