不同转移潜能肝细胞肝癌细胞系索拉菲尼药物敏感性研究模型建立

Establishment of drug sensitivity model of Sorafenib in hepatocellular carcinoma cell lines with different metastatic potential

目的:探讨索拉菲尼在不同转移潜能肝细胞肝癌(hepatocellular carcinoma,HCC)细胞系及荷HCC裸鼠移植瘤中的药物敏感性,建立HCC索拉菲尼药物敏感性研究模型。方法:通过ATP抗癌药物敏感性实验检测索拉菲尼在HCC细胞株SMMC-7721(惰性转移潜能)和MHCC-97H(高转移潜能)及对应荷HCC裸鼠移植瘤中的药物敏感性。结果:对照敏感性判定标准,SMMC-7721细胞株组索拉菲尼IC50值显著低于MHCC-97H细胞株组。荷SMMC-7721裸鼠移植瘤组索拉菲尼高度敏感,荷MHCC-97H裸鼠移植瘤组索拉菲尼中度敏感。SMMC-7721细胞株及对应裸鼠移植瘤组,索拉菲尼药物化疗敏感指数(chemotherapy sensibility index,CSI)及AUC值均优于MHCC-97H细胞株及对应裸鼠移植瘤组。SMMC-7721细胞株及对应裸鼠移植瘤组中,索拉菲尼药物生长抑制率均高于MHCC-97H组。结论:索拉菲尼在惰性转移潜能SMMC-7721细胞株和高转移潜能MHCC-97H细胞株,尤其在对应裸鼠移植瘤内,药物敏感性、CSI及AUC值、生长抑制率有差别。不同转移潜能HCC细胞系索拉菲尼药物敏感性研究模型初步建立成功。

Objective:To investigate the drug sensitivity of Sorafenib in hepatocellular carcinoma(HCC)cell lines with different metastatic potential and in HCC xenografts generated in nude mice and to establish a drug sensitivity model of Sorafenib in HCC.Methods:The drug sensitivity of Sorafenib in HCC cell lines SMMC-7721(low-metastatic potential)and MHCC-97H(high-metastatic potential)and corresponding HCC xenografts generated in nude mice was detected by ATP anti-cancer drug sensitivity assay.Results:According to the sensitivity criterion,the IC50 value of Sorafenib in SMMC-7721 cell line group was significantly lower than that in MHCC-97H cell line group.Sorafenib was highly sensitive in SMMC-7721 xenograft group and moderately sensitive in MHCC-97H xenograft group.The chemotherapy sensibility index(CSI)and AUC values of Sorafenib in SMMC-7721 cell line group and the corresponding xenograft group were both better than those in MHCC-97H cell line and the corresponding xenograft groups,respectively.The growth inhibition rate of Sorafenib in SMMC-7721 cell line and corresponding xenograft groups was higher than that in MHCC-97H groups.Conclusion:Sorafenib exhibited varying drug sensitivity,CSI and AUC values,as well as growth inhibition rates in the SMMC-7721 cell line with low-metastatic potential and the MHCC-97H cell line with high-metastatic potential,particularly evident in the respective xenograft models established in nude mice.The drug sensitivity model of Sorafenib in HCC cell lines with different metastatic potential was successfully established.