普通烟草NtZIP基因家族鉴定及镉胁迫下其表达分析

Identification of the NtZIP gene family and expression pattern analysis under cadmium stress in tobacco

植物锌铁转运蛋白ZIP主要参与调节必需金属元素的动态平衡,也参与镉(Cd)等重金属的吸收和转运。本研究运用生物信息学方法,从普通烟草(Nicotiana tabacum)基因组中共鉴定出33个NtZIP基因家族成员,其中11个成员定位在7条染色体上。亚细胞定位预测表明,26个NtZIP蛋白定位于细胞膜上,7个NtZIP蛋白定位于液泡膜上。通过邻接法,将33个NtZIP家族成员分为4个亚家族。启动子分析表明,NtZIP家族基因启动子区都含有非生物胁迫和激素相关的顺式作用元件。Cd胁迫下,荧光定量PCR结果表明,烟草NtZIP基因家族有9个成员在根中对Cd显著应答,与转录组数据基本一致,但随着Cd浓度的改变,NtZIP家族成员也出现了差异化表达,推测在烟草中NtZIP参与Cd的吸收与转运。

The plant zinc-iron transporter protein,ZIP,is mainly involved in regulating the dynamic balance of essential metal elements,as well as participating in the absorption and transport of heavy metals such as cadmium(Cd).In this study,a total of 33 members of the NtZIP gene family were identified from the ge-nome of common tobacco(Nicotiana tabacum)based on a bioinformatics approach,of which 11 mem-bers were localized on seven chromosomes.Subcellular localization prediction shows that 26 NtZIP pro-teins were localized to the cell membrane and seven NtZIP proteins to the vacuole membrane.The 33 NtZIP members were classified into four subfamilies by the neighbour-joining method.Promoter analysis shows that the promoter regions of the NtziP genes all contain abiotic stress and hormone-related cis-act-ing elements.Under Cd stress,quantitative real-time PCR results show that nine NtZIP genes are signifi-cantly responded to Cd in tobacco roots,which is basically consistent with the transcriptome results,but with the change of Cd concentration,NtZIP family members were differentially expressed,and it was hypothesized that NtZIP is involved in Cd uptake and translocation in tobacco.