红景天对尾吊小鼠眼压、视网膜结构及PI3K/AKT/HIF⁃1α信号通路的影响

Effects of Rhodiola on Intraocular Pressure,Retinal Structure and PI3K/AKT/HIF⁃1αSignaling Pathway in Tail⁃suspended Mice

针对微重力环境导致的航天相关眼综合征存在眼压升高与视网膜病变问题,研究红景天对尾吊小鼠眼压、视网膜结构的影响及作用机制。实验取55只C57小鼠,分为5组,每组11只,经2周造模,4周给药干预,历时6周;第2、4、6周测量小鼠眼压,6周后HE染色观察小鼠视网膜结构,RT⁃PCR测定小鼠视网膜内PI3K、AKT、HIF⁃1α基因表达水平。结果显示:尾吊模拟微重力状态下,小鼠眼压4周显著性高(P<0.01),6周时出现自然回落;6周小鼠视网膜结构未发生明显病理性改变,视网膜内PI3K、AKT、HIF⁃1α基因表达水平显著升高(P<0.05);应用红景天干预,可有效降低尾吊小鼠4周时的眼压(P<0.01),调节6周时视网膜内PI3K、AKT、HIF⁃1α基因表达水平(P<0.05);高剂量红景天干预造成6周时小鼠眼压较空白组显著升高(P<0.05)。红景天可降低尾吊小鼠的眼压升高,调节小鼠PI3K/AKT/HIF⁃1α信号通路表达水平,低剂量红景天干预安全性更佳。

In view of the problems of increased intraocular pressure and retinopathy caused by space⁃related eye syndrome in microgravity environment,the effects and mechanisms of Rhodiola on intraocular pressure and retinal structure in tail⁃suspended mice were studied.In the experiment,55 C57 mice were divided into 5 groups,with 11 mice in each group,and were modeled for 2 weeks and administered for 4 weeks,which lasted for 6 weeks.The intraocular pressure of mice was meas⁃ured at the 2nd,4th and 6th weeks,and the retinal structure of mice was observed by HE staining after 6 weeks,and the expression levels of PI3K,AKT and HIF⁃1αgenes in the retina of mice were measured by RT⁃PCR.The results showed that the intraocular pressure of mice was significantly higher at 4 weeks(P<0.01)and decreased naturally at 6 weeks,and there were no obvious patho⁃logical changes in the retinal structure of mice at 6 weeks,and the expression levels of PI3K,AKT and HIF⁃1αgenes in the retina were significantly increased(P<0.05),and the intraocular pressure of mice with Rhodiola intervention could be effectively reduced at 4 weeks(P<0.01),and PI3K,AKT,and the expression level of HIF⁃1αgene(P<0.05)was significantly higher at 6 weeks com⁃pared with that in the blank group(P<0.05).It is proved that Rhodiola can reduce the increase of intraocular pressure and regulate the expression level of PI3K/AKT/HIF⁃1αsignaling pathway in mice,and the safety of low⁃dose Rhodiola intervention is better.