青蒿提取物舒缓与控油祛痘功效研究

Study on the soothing, oil control and anti-acne effects of Artemisia annua extract

探讨青蒿提取物的舒缓与控油祛痘功效及作用机制。采用透明质酸酶生化反应检验青蒿提取物的舒缓功效,利用细胞模型考察青蒿提取物舒缓与控油效果,使用悬液定量法研究青蒿提取物对痤疮丙酸杆菌的抑菌效果。结果表明,青蒿提取物可有效抑制透明质酸降解(P<0.05);青蒿提取物可显著抑制RAW264.7细胞分泌炎症因子TNF-α、IL-6(P<0.05);青蒿提取物在体积分数0.5%、0.25%、0.125%时可显著抑制SZ95细胞分泌油脂(P<0.05);青蒿提取物对痤疮丙酸杆菌最小抑菌浓度是0.625%,对痤疮丙酸杆菌抑菌率随着青蒿提取物浓度增加而升高。青蒿提取物可通过舒缓与控油达到祛痘功效,且该功能可能通过减少透明质酸降解、抑制炎症因子TNF-α和IL-6产生的炎症通路,抑制油脂分泌以及抑制痤疮丙酸杆菌的生长共同实现。

Preliminary exploration of the soothing,oil control,and anti-acne effects and mechanisms of Artemisia annua extract were conducted in this study.The soothing effect of Artemisia annua extract was tested by hyaluronidase biochemical reaction.The soothing and oil-controlling effects of Artemisia annua extract were investigated by cell model.The inhibitory effect of Artemisia annua extract on propionibacterium acnes was studied by suspension quantitative method.Results showed that Artemisia annua extract could effectively inhibit the degradation of hyaluronic acid(P<0.01).Artemisia annua extract significantly inhibited the secretion of inflammatory cytokines TNF-αand IL-6 in RAW264.7 cells(P<0.05).Artemisia annua extract at 0.5%,0.25%,0.125%could significantly inhibit the secretion of oil by SZ95 cells(P<0.05).The minimum inhibitory concentration of Artemisia annua extract against propionibacterium acnes was 0.625%,and the inhibitory rate against propionibacterium acnes increased with the increase of the concentration of Artemisia annua extract.In summary,Artemisia annua extract can achieve anti-acne efficacy through soothing and oil control,and this function may be achieved by reducing hyaluronic acid degradation,inhibiting inflammatory pathways produced by inflammatory factors TNF-αand IL-6,inhibiting oil secretion,and inhibiting the growth of propionibacterium acnes.