丙型病毒性肝炎肝癌患者丙型肝炎病毒-RNA载量与甲胎蛋白、癌胚抗原的关系

Relationship between hepatitis C virus-RNA load andα-fetoprotein,carcinoembryonic antigen in patients with viral hepatitis C liver cancer

目的探讨丙型病毒性肝炎肝癌患者丙型肝炎病毒(HCV)-RNA载量与甲胎蛋白(AFP)、癌胚抗原(CEA)的关系。方法选取37例丙型病毒性肝炎肝癌患者,使用全自动化学发光测定仪检测血清CEA、AFP水平,采用荧光定量聚合酶链反应法检测HCV-RNA载量,根据HCV-RNA载量检测结果分为HCV高载量组(n=20)和HCV低载量组(n=17),比较两组患者的CEA、AFP水平。采用Spearman相关性分析法分析HCV-RNA载量与CEA、AFP的相关性。采用受试者工作特征(ROC)曲线及曲线下面积(AUC)分析CEA、AFP单独及联合检测对HCV-RNA高载量丙型病毒性肝炎肝癌的诊断价值。结果HCV-RNA高载量组患者CEA、AFP水平均高于HCV-RNA低载量组,差异均有统计学意义(P﹤0.05)。HCV-RNA载量与CEA、AFP水平均呈正相关(r=0.321、0.284,P﹤0.05)。ROC曲线显示,CEA和AFP联合检测诊断HCV-RNA高载量丙型病毒性肝炎肝癌的AUC最大。结论HCV-RNA高载量丙型病毒性肝炎肝癌患者的CEA、AFP水平较高,且HCV-RNA载量与CEA、AFP水平呈正相关。CEA和AFP联合检测对HCV-RNA高载量丙型病毒性肝炎肝癌具有一定的诊断价值。

Objective To investigate the relationship between hepatitis C virus(HCV)-RNA load andα-fetoprotein(AFP),carcinoembryonic antigen(CEA)in patients with viral hepatitis C liver cancer.Method A total of 37 patients with viral hepatitis C liver cancer were selected,the serum CEA and AFP levels were detected by automatic chemiluminescence analyzer,the HCV-RNA load was detected by fluorescence quantitative polymerase chain reaction method.All patients were divided into HCV-RNA high load group(n=20)and HCV-RNA low load group(n=17)according to the HCV-RNA load detection results,and the CEA and AFP levels of the two groups were compared.The correlation between HCV-RNA load and CEA,AFP was analyzed by Spearman correlation analysis.The diagnostic value of CEA and AFP single and combined detection for viral hepatitis C liver cancer patients with HCV-RNA high load was analyzed by receiver operating characteristic(ROC)and area under the curve(AUC).Result The CEA and AFP levels in the HCVRNA high load group were higher than those in the HCV-RNA low load group,and the differences were statistically significant(P<0.05).HCV-RNA load was positively correlated with CEA and AFP levels(r=0.321,0.284,P<0.05).ROC curve showed that the AUC of CEA and AFP combined detection in the diagnosis of viral hepatitis C liver cancer with HCV-RNA high load was the largest.Conclusion The CEA and AFP levels are high in viral hepatitis C liver cancer patients with HCV-RNA high load,and the HCV-RNA load is positively correlated with CEA and AFP levels.The combined detection of CEA and AFP has a certain value in the diagnosis of viral hepatitis C liver cancer with HCV-RNA high load.