幽门螺杆菌临床菌株四环素耐药性及其16S rRNA基因突变分析

Tetracycline resistance and 16S rRNA gene nmutation analysis of clinical strains of Helicobacter pylori

目的探讨幽门螺杆菌对四环素的耐药性,对四环素耐药菌株进行耐药基因突变位点分析,为临床根除幽门螺杆菌选择敏感抗生素以及为患者个体化治疗提供参考。方法分离培养胃黏膜组织中的幽门螺杆菌,采用琼脂稀释法进行幽门螺杆菌四环素药物敏感性检测,聚合酶链反应(PCR)法扩增四环素耐药菌株的16S rRNA基因,测序并对其基因突变位点进行分析。结果成功分离幽门螺杆菌82株,四环素耐药菌株5株,耐药率为6%。本地区幽门螺杆菌的四环素耐药基因为AGA926-928CGA的单碱基突变,另外2株耐药菌株分别发生了G970A的突变及A1119G的突变。结论本地区幽门螺杆菌临床菌株四环素耐药率较低。16S rRNA基因中的A926C突变与幽门螺杆菌对四环素耐药有关。

Objective To explore tetracycline resistance of Helicobacter pylori and analyze the mutation sites of resistance genes in tetracycline-resistant strains,and to provide guidance for selecting sensitive antibiotics for the clinical eradication of Helicobacter pylori and offer personalized treatment options for patients.Methods Helicobacter pylori was isolated and cultured from gastric mucosal tissues.The agar dilution method was used to test the tetracycline drug sensitivity of Helicobacter pylori.Polymerase chain reaction was used to amplify the 16S rRNA gene of tetracycline-resistant strains,followed by sequencing and analysis of gene mutation sites.Results A total of 82 clinical strains of Helicobacter pylori were successfully isolated,including 5 tetracycline resistant strains with a resistance rate of 6%.The tetracycline resistance gene in the study region was characterized by a single-base mutation of AGA926-928CGA in Helicobacter pylori.Additionally,two resistant strains showed mu-tations of G970A and A1119G,respectively.Conclusion The clinical strains of Helicobacter pylori in this region have a low resistance rate to tetracycline.The A926C mutation in the 16S rRNA gene is associated with Heli-cobacter pylori resistance to tetracycline.