TRMT61A的表达在头颈部鳞状细胞癌中的作用及临床价值

The role and clinical value of TRMT61A expression in head and neck squamous cell carcinoma

目的:探究tRNA甲基转移酶61A(tRNA methyltransferase 61A,TRMT61A)表达在头颈部鳞状细胞癌(head and neck squamous cell carcinoma,HNSCC)中的作用及其临床价值。方法:利用TCGA数据库对TRMT61A在泛癌中的表达水平进行分析,并对HNSCC组织及正常头颈组织中TRMT61A的mRNA表达水平及预后特征进行分析。采用Western blot实验、CCK-8实验、平板克隆实验检测敲低TRMT61A后对HNSCC细胞系的细胞增殖、克隆形成能力。结果:TRMT61A在HNSCC组织中高表达,且高表达使患者的总生存期(overall survival,OS)、无进展生存期(progression-free survival,PFS)、疾病特异性生存期(disease-specific survival,DSS)显著降低。体外实验表明敲低TRMT61A能够抑制HNSCC细胞的细胞增殖与克隆形成能力。结论:TRMT61A在HNSCC的肿瘤进展中具有重要作用,TRMT61A的表达是影响HNSCC细胞增殖的重要途径,有望成为预测HNSCC患者预后的有效指标之一,可能成为HNSCC潜在的治疗靶点。

Objective:Exploring the role and clinical value of tRNA methyltransferase 61A(TRMT61A)expression in head and neck squamous cell carcinoma(HNSCC).Methods:This study analyzed the expression levels of TRMT61A across various cancer tissues and specifically in HNSCC tissues compared to normal head and neck tissues using the Cancer Genome Atlas(TCGA)database.The expression level and prognostic characteristics of TRMT61A in patients with HNSCC were investigated.Furthermore,the proliferation and colony-formation ability of an HNSCC cell line following TRMT61A knockdown was assessed using Western blot,cell counting kit-8(CCK-8),and colony-formation assays.Results:TRMT61A was highly expressed in HNSCC tissues,and its elevated expression significantly correlated with reduced overall survival(OS),progression-free survival(PFS),and disease-specific survival(DSS)in patients.In vitro experiments demonstrated that knocking down TRMT61A suppressed proliferation and the colony-forming ability of HNSCC cells.Conclusions:TRMT61A plays an important role in tumor progression in HNSCC.Targeting TRMT61A expression is a promising approach to affect tumor cell proliferation in HNSCC and is expected to become an effective prognostic indicator for patients with HNSCC.Thus,TRMT61A is a potential therapeutic target for the treatment of HNSCC.