不同分子量黑参多糖对皮肤保湿及光损伤保护作用

Skin moisturization and photoprotective effects of black ginseng polysaccharides with different molecular weights

以黑参多糖为研究对象,分析两种黑参多糖HSP-1和HSP-2的结构,研究二者对皮肤保湿以及光损伤保护作用的效果。结果表明,HSP-1和HSP-2均含有蛋白质、均具有多糖特征峰,一级结构一致,得率HSP-1<HSP-2,分子量HSP-1<30 kDa、HSP-2>30 kDa。在单糖构成上,HSP-1以半乳糖和鼠李糖为主要单糖成分,HSP-2以阿拉伯糖为主要单糖成分。HSP-1具有三螺旋结构、HSP-2不具有三螺旋结构,二者的空间结构不一致。通过对比吸湿能力、保湿能力以及小鼠皮肤的厚度色度、羟脯氨酸、糖胺多糖和脂质含量以及总抗氧化能力、超氧化物歧化酶和丙二醛含量,发现HSP-1的吸湿保湿、抑制皮肤羟脯氨酸和脂质流失、糖胺多糖累积以及减缓氧化应激产生的炎症状态的能力均优于HSP-2。因此HSP-1多糖对皮肤保湿及光损伤保护的作用效果较佳。

The structures of two types of polysaccharides HSP-1 and HSP-2 from black ginseng and their effects on skin moisturizing and photoprotection were studied.The results showed that both HSP-1 and HSP-2 contain proteins and had characteristic polysaccharide peaks,with identical primary structures.The yield of HSP-1 was less than HSP-2,the molecular weight of HSP-1 was less than 30 kDa,and HSP-2 molecular weight was more than 30 kDa.In terms of monosaccharide composition,galactose and rhamnose were the main monosaccharide components of HSP-1,and arabinose was the main monosaccharide component of HSP-2.HSP-1 had a triple helix structure,while HSP-2 lacked this feature,and indicating their spatial structure was different.By comparing their moisture absorption ability,moisture retention ability,skin thickness and chroma,hydroxyproline,glycosaminoglycan and lipid content,total antioxidant capacity,superoxide dismutase and malondialdehyde content of mouse skin,it was found that HSP-1 was better than HSP-2 in moisture absorption and moisture retention,inhibition of skin hydroxyproline and lipid loss,accumulation of glycosaminoglycan,and alleviation of inflammatory state caused by oxidative stress.Therefore,HSP-1 polysaccharide could have a better effect on skin moisturizing and photoprotection.