基于eDNA和形态学的九江市城市湖泊浮游动物分析

Zooplankton in urban lakes of Jiujiang city based on eDNA and morphology

利用环境DNA监测并量化水体中生物多样性是近年水生生态学中关键的技术进步之一,该技术不仅提供快速准确的群落鉴定结果和物种多样性信息,还可以用于物种的相对丰度评估。浮游动物在水生食物链中起着能量和物质传递的作用,且能快速及时反映生态环境状况。基于八里湖、赛城湖浮游动物的eDNA和形态学鉴定方法,对浮游动物种类组成、生物多样性、相对丰度和群落结构特征等进行探究分析。结果表明:eDNA鉴定方法能比形态学鉴别出更多的类群,其具备鉴定深度更高的优势,但也存在误检假阳性风险;在生物多样性的表征上,两种方法结果存在部分显著差异。

Monitoring and quantifying biodiversity in water bodies using environmental DNA is one of the key technological advances in aquatic ecology in recent years.Environmental DNA technology not only provides rapid and accurate community identification results and species diversity information,but also can be used for relative abundance assessment of species.Zooplankton play a role in energy and material transfer in the aquatic food chain,and can quickly and timely reflect the ecological environment.Based on the eDNA and morphological identification methods of zooplankton in Bali lake and Saicheng lake,the species composition,biodiversity,relative abundance,and community structure characteristics of zooplankton were explored and analyzed.The results showed that the eDNA identification method identified more taxa than morphological identification,with the advantage of higher identification depth,but also had the risk of false positives;there were some significant differences in the results of the two methods in terms of biodiversity representation.