稳定同位素标记-高效液相色谱-串联质谱法测定动物源性食品中12种利尿剂

Simultaneous determination of 12 diuretics in animal-derived foods by ultra-high performance liquid chromatography-tandem mass spectrometry with stable isotopic labeling

本研究建立了稳定同位素标记-高效液相色谱串联质谱法测定动物源性食品中12种利尿剂的方法。试样经5%甲酸乙腈提取,通过式固相萃取柱净化,采用液相色谱-质谱/质谱仪测定,同位素内标法定量。12种利尿剂在各自浓度范围内线性关系良好,平均回收率介于71.3%~104.7%,相对标准偏差介于1.2%~11.1%(n=6)。该方法具有灵敏度高、准确性好、操作简单、检测效率高等特点,能够满足多种动物源性食品中12种利尿剂的检测需求。

This study establishes a method for the determination of 12 diuretics in animal derived foods by stable isotope labelinghigh performance liquid chromatography-tandem mass spectrometry.The sample was extracted with 5%formic acid in acetonitrile,and the extract was purified by pass-through solid phase extraction before being measured by liquid chromatography-tandem mass spectrometry.The internal standard method was used for quantification.The linear relationship of 12 diuretics was good within their respective concentration ranges.The average recoveries ranged from 71.3%to 104.7%,and the relative standard deviations were between 1.2%and 11.1%(n=6).This method has the characteristics of high sensitivity,good accuracy,simple operation and high detection efficiency.It can meet the detection needs of 12 diuretics in animal-derived food.