厚朴酚对实验性牙周炎大鼠治疗作用的研究

Therapeutic effect of magnolol on experimental periodontitis in rats

目的观察厚朴酚对实验性牙周炎大鼠模型的治疗作用。方法将50只3月龄雄性SD大鼠随机分为空白组、模型组、局部治疗组、厚朴酚组、局部治疗+厚朴酚组,每组10只。除空白组外,其余组予钢丝结扎上颌第一磨牙建立实验性牙周炎模型,模型建立4周后,按照分组分别进行相应的治疗8周,进行口腔检查,股动脉放血处死大鼠,制作牙周组织切片,苏木精-伊红(HE)染色观察牙周组织变化,免疫组化法检测牙周组织中肿瘤坏死因子α(TNF-α)、基质金属蛋白酶8(MMP-8)表达情况。结果厚朴酚配合牙周局部治疗能够显著改善牙周组织的破坏情况,降低牙周组织中的TNF-α、MMP-8表达,对大鼠实验性牙周炎起到了很好的治疗作用。结论厚朴酚对实验性牙周炎治疗效果明显,并且能够显著降低牙周组织中的炎症细胞因子,促进牙周病变愈合,促进牙槽骨的新生。

Objective To study the therapeutic effect of magnolol on experimental periodontitis in rats.Methods Fifty 3-month-old male SD rats were selected and randomly divided into 5 groups:control group(Group A),experimental periodontitis group(Group B1),local treatment group(Group B2),experimental periodontitis treatment group(Group B3),and local treatment with magnolol(Group B4),with 10 rats in each group.An experimental periodontitis model was established by ligating the maxillary first molar with steel wire.After 4 weeks of establishment,the model was treated according to the corresponding groups for 8 weeks.Oral examination was conducted,and rats were sacrificed by bleeding from the femoral artery.Periodontal tissue slices were made,and changes in periodontal tissue were observed by hematoxylin-eosin(HE)staining.The expression of tumor necrosis factor-α(TNF-α)and matrix metalloproteinase-8(MMP-8)in periodontal tissue were detected by immunohistochemistry.Results The combination of magnolol and local periodontal treatment can significantly improve the damage of periodontal tissue and levels of TNF-αand matrix MMP-8,with a good therapeutic effect on experimental periodontitis in rats.Conclusion Magnolia officinalis has a significant therapeutic effect on experimental periodontitis,and it can significantly reduce inflammatory cytokines in periodontal tissue,promote periodontal disease healing,and alveolar bone regeneration.