巴马火麻油对大鼠抗氧化功能的影响

Effect of Anti-oxidative Function of Bama Hemp Seed Oil

为研究巴马火麻油对大鼠抗氧化功能的影响,依据《关于印发抗氧化功能评价方法等9个保健功能评价方法的通知》(国食药监保化[2012]107号)附件1抗氧化功能评价方法,采用巴马火麻油对成年大鼠进行实验,设溶媒对照组、空白对照组及火麻油高、中、低3个剂量组,连续灌胃30 d。结果表明,受试样品组肝组织匀浆和血清中的丙二醛低于溶媒对照组,差异显著(P<0.05),肝组织匀浆中的超氧化物歧化酶活性极显著高于溶媒对照组(P<0.01);受试样品组肝组织匀浆和血清中的还原型谷胱甘肽含量高于溶媒对照组,差异极显著(P<0.01)。这表明在本实验条件下,动物实验的脂质过氧化产物、抗氧化酶、抗氧化物质3项指标阳性,表明火麻油具有抗氧化功能。

In order to study the anti-oxidative function of Bama hemp seed oil,adult rats are tested with Bama hemp seed oil according to the antioxidant function evaluation method of annex 1 of the Notice on the Issuance of 9 Health Function Evaluation Methods including Antioxidant Function Evaluation Methods(2012)107 of China Food and Drug Administration.Solvent control group,blank control group and sesame oil high-dose,medium-dose and low-dose groups are set up for continuous gavage for 30 days.The results show that the malondialdehyde(MDA)levels in liver homogenate and serum in the test group are significantly lower than those in the solvent control group(P<0.05),and the superoxide dismutase(SOD)activity in liver homogenate is significantly higher than that in the solvent control group(P<0.01).The contents of liver homogenate and serum reducing glutathione(GSH)in the test group are significantly higher than those in the solvent control group(P<0.01).These results showed that the lipid peroxidation products,antioxidant enzymes and antioxidant substances in animal experiments are positive under the experimental conditions,indicating that Bama hemp seed oil has antioxidant function.