微波消解-电感耦合等离子体质谱法测定食用菌中的重金属含量

Determination of Heavy Metal Content in Edible Fungi by Microwave Digestion Inductively Coupled Plasma Mass Spectrometry

利用微波消解-电感耦合等离子体质谱仪法建立同时测定食用菌中砷(As)、铅(Pb)、镉(Cd)、铬(Cr)和汞(Hg)含量的方法。在测食用菌样品中As、Pb、Cd和Cr的质量浓度为0.5~20.0μg·L^(-1),Hg的质量浓度为0.05~2.00μg·L^(-1)时,线性相关系数(r)均大于或等于0.9991,检出限分别为0.0010、0.0010、0.0020、0.0020、0.0003 mg·kg^(-1)。其中,Pb、Cd、Cr、As和Hg的加标回收率分别为93.5%~100.0%、94.2%~98.6%、97.00%~101.4%、90.1%~99.5%和90.0%~99.5%,相对标准偏差分别为0.86%~1.58%、0.18%~6.45%、0.39%~3.77%、0.27%~1.98%和0.39%~3.27%。说明该方法具有操作简单、重复性好、灵敏度高等优点,可以适用于食用菌中的As、Pb、Cd、Cr、Hg共5种重金属含量的分析检测。

The test established a method for simultaneous determination of As,Pb,Cd,Cr and Hg content in edible fungi using microwave digestion inductively coupled plasma mass spectrometry.As,Pb,Cd and Cr in edible fungi at mass concentrations of 0.5-20.0μg·L^(-1)and Hg at a mass concentration of 0.05-2.00μg·L^(-1),the linear correlation coefficients(r)are all greater than or equal to 0.9991,and the detection limits are 0.0010,0.0010,0.0020,0.0020,and 0.0003 mg·kg^(-1),respectively.Among them,the spiked recoveries of Pb,Cd,Cr,As,and Hg are 93.5%-100.0%,94.2%-98.6%,97.00%-101.4%,90.1%-99.5%,and 90.0%-99.5%,respectively.The relative standard deviations are 0.86%-1.58%,0.18%-6.45%,0.39%-3.77%,0.27%-1.98%,and 0.39%-3.27%,respectively.Thus,this method has the advantages of simple operation,good repeatability,and high sensitivity,and can be applied to the analysis and detection of As、Pb、Cd、Cr、Hg in edible fungi.