食用菌中18种有机氯类农药残留物的检测方法验证

Validation of the Detection Methods of 18 Organochlorine Pesticide Residues in Edible Fungi

使用改进的QuEChERS方法进行前处理,利用气相色谱法分析测定了食用菌中18种有机氯类农药残留物,通过标准曲线、检出限和定量限,食用菌样品空白加标后测定回收率,计算精密度等进行方法学验证。结果表明,18种有机氯类农药标准曲线线性良好,相关系数R2为0.99175~0.99998,加标回收率为69.1%~91.5%,相对标准偏差范围为0.7%~4.1%,检出限和定量限均能满足有机氯类农药残留的最大限量检测要求。

18 organicchlorine pesticide residues in edible fungi were analyzed and determined by gas chromatography using an improved QuEChERS method.The method was validated by standard curves,detection limit and quantification limi,blank addition of edible fungi samples,determination of recovery rate,and precision calculation.The results showed that the standard curves of 18 organochlorine pesticides had good linearity,with a correlation coefficient R2 of 0.99175-0.99998,a recovery rate of 69.1%-91.5%,and a relative standard deviation(RSD)range of 0.7%-4.1%.Both the detection limit and quantification limit can meet the maximum limit detection requirements for organochlorine pesticide residues.