超高效液相色谱(UPLC)用于茯苓水溶性成分特征图谱研究

Study on UPLC Fingerprint Chromatogram of Water-soluble Chemical Constituent in Poria Cocos

运用超高效液相色谱法(UPLC)建立茯苓水溶性成分特征图谱,以期为茯苓质量控制提供参考。采用HSS T3-C_(18)(2.1 mm×100 mm,1.8μm)色谱柱;以乙腈-0.1%磷酸梯度洗脱;检测波长为243 nm;流速为0.3 mL/min,柱温30℃。通过对12批茯苓样品的测定,标定8个共有峰,并指认其中4个分别为去氢土莫酸、猪苓酸C、3-表去氢土莫酸、去氢茯苓酸。该方法准确可靠、重复性好,为更好地控制茯苓内在质量,提供了科学依据。

A UPLC characteristic chromatographic profile analysis method was established to quickly assess water-soluble chemical constituent quality and provide basis fro controlling Poria quality.UPLC characteristic chromatographic profiles of twelve batches of Poria were determined by UPLC,with HSS T3-C_(18)column(2.1 mm×100 mm,1.8μm)eluted with the gradient elution solvent system composed of water containing 0.1%phosphoric acid and acetonitrile in gradient mode.The detection wavelength was set at 243 nm,The flow rate was 0.3 mL/min,the column temperature was maintained at 30℃.The common mode of UPLC characteristic chromatographic profile was set up,there were eight common peaks,four of which were identified.The method was so time-saving that it can be used for the quality control of Poria.