子宫内膜异位症患者异位内膜组织中Ninj1、IL-1β及PGP-9.5的表达及意义

Expression and significance of Ninj1,IL-1βand PGP-9.5 in ectopic endometrial tissues of patients with endometriosis

目的探讨子宫内膜异位症(endometriosis,EMT)患者的异位内膜组织中神经损伤诱导蛋白1(Ninj1)、白细胞介素-1β(IL-1β)及蛋白基因产物9.5(PGP-9.5)的表达及与痛经的关系。方法以2020年4月—2021年4月在我院行妇科术后病理检查确诊为卵巢EMT患者的异位内膜组织40例为A组,并获取其中20例患者的在位子宫内膜组织为B组,收集同期20例子宫肌瘤患者的正常子宫内膜组织作为C组。根据有无痛经将A组分为痛经亚组与非痛经亚组,每组20例。采用免疫组织化学方法检测A、B、C组内膜组织中Ninj1、IL-1β及PGP-9.5的表达,并分析其相关性;采用酶联免疫吸附试验检测A组与C组患者外周血中IL-1β的表达水平。结果A组患者外周血中IL-1β的表达水平显著高于C组(t=-4.184,P<0.05);与C组相比,A、B组内膜组织中3种蛋白阳性表达构成比均明显升高(χ^(2)=4.286~19.394,P<0.05),与B组相比,A组内膜组织中3种蛋白阳性表达构成比明显升高(χ^(2)=4.149~5.272,P<0.05)。痛经亚组内膜组织中Ninj1与PGP-9.5蛋白阳性表达构成比明显高于非痛经亚组(χ^(2)=8.533、11.905,P<0.05)。A组内膜组织中Njnj1与PGP-9.5、IL-1β的表达呈正相关(r=0.733、0.628,P<0.05)。结论Ninj1、IL-1β及PGP-9.5在异位内膜组织中阳性表达比例较高,可能参与EMT的发生与发展;痛经亚组内膜组织中Ninj1与PGP-9.5蛋白的阳性表达构成比高于非痛经亚组,可能与EMT疼痛发生机制密切相关。

Objective To investigate the expression levels of nerve injury-induced protein 1(Ninjl),interleukin-1β(IL-1β),and protein gene product 9.5(PGP-9.5)in the ectopic endometrial tissues of patients with endometriosis(EMT)and their relationship with dysmenorrhea.Methods Ectopic endometrial tissues from 40 patients with ovarian EMT diagnosed by post-ope-rative gynecological pathological examination in our hospital from April 2020 to April 2021 were selected as group A.In situ endo-metrial tissues from 20 of these patients were obtained as group B.Normal endometrial tissues from 20 patients with uterine fi-broids in the same period were collected as group C.Group A was divided into a dysmenorrhoea subgroup and a non-dysmenorrhoea subgroup according to the presence or absence of dysmenorrhoea,with 20 patients per subgroup.The expression levels of Ninj1,IL-1β,and PGP-9.5 in the endometrial tissues of groups A,B,and C were measured by immunohistochemistry,and their correlation was analyzed.The expression level of IL-1βin the peripheral blood of patiens in groups A and C was measured by enzyme-linked immunosorbent assay.Results The expression level of IL-1βin the peripheral blood of patients in group A was signifi-cantly higher than that in group C(t=-4.184,P<0.05).The positive expression rates of three proteins in the endometrial tissues were significantly increased in groups A and B compared with group C(χ^(2)=4.286-19.394,P<0.05)and in group A compared with group B(χ^(2)=4.149-5.272,P<0.05).The positive expression rates of Ninjl and PGP-9.5 proteins in the endometrial tissues were significantly higher in the dysmenorrhoea subgroupthan in the non-dysmenorrhoea subgroup(χ^(2)=8.533,11.905,P<0.05).The expression of Ninj1 was positively correlated with the expression of PGP-9.5 and IL-1βin the endometrial tissues of group A(r=0.733,0.628,P<0.05).Conclusion Ninjl,IL-1β,and PGP-9.5 are highly expressed in the ectopic endometrial tissues,which may be involved in the occurrence and development of EMT.The positive expression rates of both Ninjl and PGP-9.5 in the endometrial tissues of the dysmenorrhoea subgroup were higher than those in the non-dysmenorrhoea subgroup,which may be closely related to the mechanism of EMT pain.