蜂蜜中链霉素仿生印迹电化学传感检测方法

Biomimetic imprinted electrochemical sensing detection of streptomycin in honey

动物源食品中链霉素残留危害严重,建立高性能分析方法是确保食品安全和人类健康的必要手段。仿生印迹电化学传感检测方法具有高效、特异和灵敏等优点,已成为食品安全检测技术研究的重要领域。该研究以链霉素为模板分子,氧化石墨烯和壳聚糖为增敏材料,3-氨基苯基硼酸和邻苯二胺为复合功能单体,采用电聚合法构建了仿生印迹聚合物传感界面,建立了蜂蜜中链霉素的仿生印迹电化学传感检测方法。通过扫描电镜对印迹传感界面进行了表征,采用循环伏安法、差分脉冲伏安法和电化学交流阻抗法对仿生印迹传感器的电化学性能进行表征。在最佳反应条件下,该传感器的检测限为2.59×10^(-12) mol/L,线性范围为1.0×10^(-10)~1.0×10^(-6) mol/L,洋槐蜜、枣花蜜和桂花蜜样品的加标回收率分别为94.78%~106.24%、93.65%~103.81%和97.19%~106.18%,相对标准偏差≤4.28%。试验结果表明,该方法具有良好的重复性、稳定性和重现性,能够满足蜂蜜中链霉素残留分析的需要。相关研究为复杂基质中痕量靶标的高特异性和超灵敏分析提供了技术支撑,对于蜂蜜中链霉素的快速筛查和安全监测具有重要意义。

There has been a serious hazard for streptomycin residues in animal-derived foods.It is necessary to establish a high-performance analytical method to ensure food safety and human health.The biomimetic imprinting electrochemical sensing detection method has the advantages of high efficiency,specificity,and sensitivity,and has become an important area of research in the field of food safety detection technology.In this study,a biomimetic imprinted sensing interface was constructed by electropolymerization with streptomycin as the template,graphite oxide and chitosan as the sensitizing materials,and 3-aminobenzeneboronic acid and O-phenylenediamine as the composite functional monomers.The biomimetic imprinted electrochemical sensing detection method was then developed and applied for STR residue analysis in honey.The imprinted interface was characterized by scanning electron microscopy,and the electrochemical performance of the proposed sensor was evaluated with cyclic voltammetry,differential pulse voltammetry,and electrochemical impedance analysis.Under the optimal reaction conditions,the linear range was calculated as 1.0×10^(-10)-1.0×10^(-6) mol/L with a detection limit of 2.59×10^(-12) mol/L,the recoveries of sophora nectar,jujube nectar,and osmanthus nectar were 94.78%-106.24%,93.65%-103.81%,and 97.19%-106.18%,respectively,and the relative standard deviation was less than or equal to 4.28%.The experimental results show that this method has good repeatability,stability,and reproducibility.The related research provides technical support for the high specificity and ultrasensitive analysis of trace target compounds in complex matrices,which is of great significance for the rapid screening and safety monitoring of streptomycin in honey.