扛板归粗多糖抗氧化、抗炎、镇痛和抑菌活性研究

Antioxidant,Anti-inflammatory,Analgesic and Antimicrobial Activities of Crude Polysaccharides from Polygonum perfoliatum L.

目的:研究扛板归粗多糖体内外抗氧化、抗炎、镇痛和抑菌活性。方法:水提醇沉法制备扛板归粗多糖。以清除1,1-二苯基-2-三硝基苯肼(1,1-Diphenyl-2-picrylhydrazyl,DPPH)自由基、羟自由基和超氧阴离子自由基的能力为体外抗氧化评价方法,以测定总超氧化物歧化酶(Total superoxide dismutase,T-SOD)活力、过氧化氢酶(Catalase,CAT)活力和丙二醛(Malondialdehyde,MDA)含量为体内抗氧化指标,综合考察粗多糖抗氧化活性;采用二甲苯诱导小鼠耳廓肿胀法,评价粗多糖抗炎效果;测定小鼠热板痛阈值评价粗多糖镇痛作用;采用牛津杯法评价粗多糖体外抑菌效果。结果:扛板归粗多糖对DPPH自由基、羟自由基和超氧阴离子自由基具有较好的清除能力,呈剂量依懒性,半抑制浓度值(IC_(50))分别为420.93、171.53和575.40μg/mL;与对照组相比,粗多糖在体内能提高组织T-SOD活力和CAT活力,降低MDA水平。粗多糖能抑制二甲苯诱导的肿胀作用,抑制率高达40.95%。但是,该粗多糖镇痛作用不明显,对大肠埃希氏菌和金黄色葡萄球菌均无抑制作用。结论:扛板归粗多糖具有抗氧化和抗炎活性,有望开发成天然抗氧化剂。

Objectives:This study aimed to investigate the in vivo and in vitro antioxidant,anti-inflammatory,analgesic,and antimicrobial activities of crude polysaccharides from Polygonum erfoliatum L..Methods:The crude polysaccharides were prepared using the water extraction and alcohol precipitation method.The in vitro antioxidant activity was assessed based on the capability to scavenge 1,1-diphenyl-2-picrylhydrazyl(DPPH)radicals,hydroxyl radicals,and superoxide anion radicals.The in vivo antioxidant indicators were the determination of total superoxide dismutase(T-SOD)activity,catalase(CAT)activity,and malondialdehyde(MDA)content,providing a comprehensive evaluation of the antioxidant activity of the crude polysaccharides.The anti-inflammatory effect of the crude polysaccharides was assessed using the xyleneinduced mice ear edema method.The analgesic effect was evaluated by determining the pain threshold in mice using the hot plate test.The in vitro antimicrobial activity was evaluated by the Oxford cup method.Results:The crude polysaccharides displayed effective scavenging abilities against DPPH radicals,hydroxyl radicals,and superoxide anion radicals,with the activity significantly dependent on the concentration of the polysaccharides.The half-maximal inhibitory concentration(IC_(50))values were 420.93,171.53,and 575.40μg/mL,respectively.Compared to the control group,the polysaccharides enhanced the activity of T-SOD and CAT,reduced MDA levels in vivo.The crude polysaccharides inhibited xylene-induced swelling with an inhibition rate reaching 40.95%.However,the analgesic effect of the crude polysaccharides was not significant,and they showed no inhibitory effects against Escherichia coli and Staphylococcus aureus.Conclusions:The crude polysaccharides from Polygonum perfoliatum L.possess antioxidant and anti-inflammatory activities and have the potential to be developed as natural antioxidants.