免疫介导的再生障碍性贫血模型小鼠巨噬细胞归巢及特征分析

Homing and characteristic analysis of macrophage in immune-mediated aplastic anemia model mice

研究免疫介导的再生障碍性贫血(AA)模型小鼠体内巨噬细胞在不同器官的动态归巢过程、特征。通过磁珠分选出供鼠淋巴结中巨噬细胞并用PKH67荧光标记,参照AA模型制备方法造模后,分析小鼠血常规、骨髓活检及HE染色结果,验证造模效果。在造模的第4、8、12天,收集骨髓、脾脏和淋巴结单个核细胞,通过流式细胞术分析PKH67荧光标志供鼠巨噬细胞的动态变化。探究PKH67荧光标志的巨噬细胞,在AA模型小鼠发病过程中的动态变化,观测到供鼠巨噬细胞归巢到淋巴结并扩增分化,最终转运至骨髓和脾脏。通过蛋白组学质谱分析,初步揭示了巨噬细胞参与AA骨髓微环境激活后的相关免疫炎症反应通路,为病理性巨噬细胞参与AA模型小鼠的发病提供了依据。

To investigate the dynamic homing process and characteristics of macrophages in different organs of immune-mediated aplastic anemia(AA)model mice.Macrophages in donor lymph nodes were sorted by magnetic beads and labeled with PKH67.After modeling according to the preparation method of the AA model,peripheral blood rountine analysis,bone marrow biopsy and HE staining results were analyzed to verify the modeling effect.On days 4,8,and 12 of modeling,the bone marrow,spleen,and lymph node mononuclear cells were collected,and dynamic changes of PKH67-labeled macrophages in donor mice were analyzed by flow cytometry.In this study,dynamic changes in PKH67-labeled macrophages in the pathogenesis of AA model mice were explored.Macrophages in donor mice homed to the lymph nodes,expanding and differentiating in the lymph nodes,and finally transported to the bone marrow and spleen.Through proteomics mass spectrometry analysis,the related immune inflammatory response pathway of macrophages involved in the activation of the AA bone marrow microenvironment was preliminarily revealed,which provides a basis for the pathological macrophages involved in the pathogenesis of AA model mice.