高溶氧水对高糖高脂模型小鼠异常血糖的保护作用

Protective Effects of Oxygen-rich Water on Abnormal Blood Glucose Levels in Mice with Hyperglycemia and Hyperlipidemia

该研究探讨了高溶氧水对高糖高脂小鼠模型血糖和血脂的影响。采用SPF级成年雄性昆明种小鼠,以高糖高脂饲料和腹腔注射四氧嘧啶的方式构建高糖高脂模型,分别用无菌饮用水、高溶氧水和二甲双胍处理30 d,定期测量小鼠体重、饮水和摄食量。处死小鼠前测量空腹血糖和口服葡萄糖耐量,计算相关脏器系数和脂肪系数、取血清测定血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)水平,检测肝脏谷胱甘肽(GSH)、丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性,并观察肝组织病理学变化。结果表明高溶氧水处理后的实验组与模型对照组相比,饮水量、摄食量、空腹血糖和口服葡萄糖耐量的曲线下面积等均显著降低(P<0.05),其中空腹血糖为8.36 mmol/L,下降率达31%,其余指标无明显变(P>0.05)。综上,高溶氧水对高糖高脂模型小鼠的异常血糖水平具有改善作用,并未增加机体的氧化应激水平,该实验结果从食品角度为辅助降血糖策略提供了新的思路和参考。

The effects of oxygen-rich water on blood glucose and lipid levels in mouse models of hyperglycemia and hyperlipidemia were investigated.The hyperglycemia and hyperlipidemia models were constructed by feeding SPF adult male Kunming mice a high-sugar and high-fat diet and administering an intraperitoneal injection of alloxan.The models were separately treated with sterile drinking water,oxygen-rich water,and metformin for 30 days.During the intervention,the body weight,water intake,and food consumption were measured at regular intervals.The fasting blood glucose levels and oral glucose tolerance were measured before the mice were euthanized.In addition,the relevant organ and fat indices were calculated,and serum total cholesterol(TC),triglyceride(TG),high-density lipoprotein cholesterol(HDL-C),and lowdensity lipoprotein cholesterol(LDL-C)levels were determined.The liver glutathione(GSH)and malondialdehyde(MDA)contents and the superoxide dismutase(SOD)activity were determined.Furthermore,histopathological changes in the liver were observed.The results revealed that compared with the control model group,the experimental group treated with oxygen-rich water demonstrated significant reductions(P<0.05)in water intake,food intake,fasting blood glucose levels,and area under the oral glucose tolerance curve.In particular,the fasting blood glucose levels equaled 8.36 mmol/L,with a decrease of 31%,while the rest of the indicators did not change significantly(P>0.05).In conclusion,oxygen-rich water has an ameliorating effect on abnormal blood glucose levels of hyperglycemic and hyperlipidemic model mice;at the same time,it does not increase the oxidative stress levels in mice.The findings of this study provide new insights and references for adjunctive hypoglycemic strategies from the perspective of food.