环状RNA Hsa_circRNA_048764对乳腺癌细胞增殖机制的影响

Effect of Circular RNA Hsa_circRNA_048764 on Proliferation Mechanism of Breast Cancer Cells

目的本研究旨在探讨GEO数据库、本院组织标本、乳腺癌细胞中明确其表达及其发挥作用的机制。方法生物信息学方法分析hsa_circRNA_048764在GSE165884中的表达。实时定量PCR检测乳腺癌组织和细胞中hsa_circRNA_048764的表达。CCK-8检测hsa_circRNA_048764对乳腺癌细胞增殖的影响。生物信息学技术预测hsa_circRNA_048764的下游基因。实时定量PCR和Western Blot法检测下游基因的表达。生物信息学分析hsa-miR-96-5p对乳腺癌预后的影响,并分析X-box结合蛋白1(the X-box binding protein 1,XBP1)与乳腺癌免疫浸润之间的关系以及对免疫治疗预后的影响。结果hsa_circRNA_048764在GSE165884数据库、乳腺癌组织以及乳腺癌细胞中高表达。敲低hsa_circRNA_048764表达后抑制乳腺癌细胞的增殖,并促进乳腺癌细胞中hsa-miR-96-5p的表达。过表达hsa-miR-96-5p后抑制XBP1蛋白的表达。XBP1与CD8+T细胞密切相关。XBP1与抗PD-L1免疫治疗患者的预后相关。结论hsa_circRNA_048764在乳腺癌组织和细胞中均高表达,敲低hsa_circRNA_048764能抑制乳腺癌细胞增殖,其分子机制可能是竞争性结合hsa-miR-96-5p,进而调控XBP1蛋白的表达,XBP1与乳腺癌CD8+T细胞浸润且与肿瘤抗PD-L1免疫治疗预后相关。

Objective The purpose of this study was to investigate the expression and mechanism of GEO in the database,tissue samples and breast cancer cells.Methods The expression of hsa_circRNA_048764 in GSE165884 was analyzed by bioinformatics methods,and the expression of hsa_circRNA_048764 in breast cancer tissues and cell lines was detected by real-time quantitative PCR.CCK-8 detects the effect of hsa_circRNA_048764 on the proliferation of breast cancer cell lines.Bioinformatics techniques were used to predict downstream genes of hsa_circRNA_048764.Real-time quantitative PCR and Western Blot were used to detect downstream gene expression.Bioinformatics was used to analyze the effect of hsa-miR-96-5p on the prognosis of breast cancer,and the relationship between the X-box binding protein 1(XBP1)and immune invasion of breast cancer,as well as the effect on the prognosis of immunotherapy.Results Hsa_circRNA_048764 was highly expressed in breast cancer tissues and breast cancer cells in GSE165884 database.Knockdown of hsa_circRNA_048764 expression inhibited the proliferation of breast cancer cells and promoted the expression of hsa-miR-96-5p in breast cancer cells.Overexpression of hsa-miR-96-5p inhibited the expression of XBP1 protein.XBP1 is closely related to CD8+T cells.XBP1 is associated with prognosis in patients treated with anti-PD-L1 immunotherapy.Conclusion Hsa_circRNA_048764 was highly expressed both in breast cancer tissues and cell lines.Knockdown of hsa_circRNA_048764 could inhibit the proliferation of breast cancer cells,and its molecular mechanism may be that it competitively binds the expression of hsa-miR-96-5p and regulates the expression of XBP1 protein.XBP1 is involved in the immune microenvironment of breast cancer and is associated with anti-PD-L1 immunotherapy.