基于响应面法结合熵权法优化浙贝母(灰贝)加工工艺

Optimization of Fritillariae Thunbergii Bulbus(Huibei)Processing Technology Based on Response Surface Method Combined with Entropy Weight Method

目的 应用响应面法结合熵权法优化浙贝母(灰贝)的加工工艺。方法 以伊贝辛、贝母辛、贝母素甲、贝母素乙、异贝母甲素为考察指标,熵权法确定各指标成分权重,在单因素试验的基础上,采用Box-Behnken响应面法设计优化浙贝母(灰贝)的加工工艺。结果 得到最优提取工艺为撞皮时间95 s、料灰比0.9%、发汗时间5 h、干燥温度45℃,验证结果与预测值偏差为0.5%。结论 响应面法优选的加工工艺稳定性好,可操作性强,可为浙贝母(灰贝)产地加工炮制一体化的质量控制提供科学依据。

OBJECTIVE To optimize the processing technology of Fritillariae Thunbergii Bulbus(Huibei)by response surface methodology combined with entropy weight method.METHODS The single factor experiment combined with Box-Behnken design was used to optimize the processing technology,with 5 kinds of alkaloid(yibeissine,peimisine,peimine,peiminine,isobeta metrin)as the inspection indicators,combined with the weight coefficients were calculated by entropy weight method.RESULTS The optimum extraction process was determined as follows:crashing time for 95 seconds,material-to-ash ratio for 0.9%,sweating time for 5 h,and drying temperature for 45℃.The RSD between the validation results and the predicted values was 0.5%.CONCLUSION This optimized processing technology is stable amd feasible,which can provide the scientific basis for quality control of Fritillariae Thunbergii Bulbus(Huibei)processing technology.