巴戟天联合运动对小鼠坐骨神经损伤修复的影响

Effects of Morinda officinalis and exercise on the repair of sciatic nerve injury in mice

目的 研究巴戟天和运动对坐骨神经损伤小鼠神功能恢复的作用。方法 建立小鼠坐骨神经损伤模型,80只小鼠随机分为对照不运动、对照运动、巴戟天不运动和巴戟天联合运动组,每组20只。巴戟天组每天按0.1 ml/10 g巴戟天水提液灌胃、对照组每天按0.1 ml/10 g生理盐水灌胃,适应性喂养5 d后,灌胃半小时后对照运动组和巴戟天联合运动组小鼠在动物跑台上跑步训练,1次/天,连续28 d。通过观察神经元变化和神经胶质细胞数量、检测小鼠血清丙二醛(MDA)含量和超氧化歧化酶(SOD)活力值、坐骨神经功能指数、腓肠肌湿重等,观察巴戟天和运动对坐骨神经功能的恢复情况。结果 各组都存在神经元浓缩、破碎,尼氏Nissl数量减少,但巴戟天联合运动组神经元浓缩、破碎的情况明显减少,神经胶质细胞数量明显增加。腓肠肌湿重结果显示,对照组与巴戟天联合运动相比差异有统计学意义(P<0.01);坐骨神经功能指数结果显示,21 d时对照组与巴戟天联合运动相比差异有统计学意义(P<0.01),巴戟天联合运动和巴戟天不运动相比差异有统计学意义(P<0.05),28 d时对照不运动与巴戟天组相比差异有统计学意义(P<0.05);MDA含量测定结果显示,对照组与巴戟天联合运动相比差异有统计学意义(P<0.05);SOD活力值检测显示,对照运动与巴戟天组相比,差异有统计学意义(P<0.01)。结论 巴戟天可能有助于小鼠坐骨神经损伤的恢复,其与运动联合的恢复效果更佳。

Objective To study the effect of Morinda officinalis and exercise on the recovery of divine func-tion in mice with sciatic nerve injury.Methods Establish a model of sciatic nerve injury in mice,with 80 mice ran-domly divided into control group without exercise,control group with exercise,group without exercise of Morinda of-ficinalis,and group with combined exercise of Morinda officinalis,with 20 mice in each group.The Morinda officina-lis group was gavaged with Morinda officinalis aqueous extract at 0.1 ml/10 g per day,and the control group was ga-vaged with saline at 0.1 ml/10 g per day,and after acclimatization feeding for 5 d,the mice of the control exercise group and the Morinda officinalis exercise group were trained to run on the animal running platform after half an hour of gavage,1 time/d for 28 consecutive days.By observing the neuronal changes and the number of neuroglial cells,detecting the serum malondialdehyde(MDA)content and superoxide dismutase(SOD)vitality value of mice,sciat-ic nerve function index,gastrocnemius muscle wet weight,etc。,to observe the recovery of sciatic nerve function by Morinda officinalis and exercise.Results There were neuronal condensation and fragmentation in all groups,and the number of Nissl was reduced,but neuronal condensation and fragmentation were significantly reduced and the number of neuroglia was significantly increased in the bacopausal exercise group.The results of gastrocnemius wet weight showed a statistically significant difference(P<0.01)between the control group compared to bacopausal exer-cise.The results of sciatic nerve function index showed that the difference between the control group and bacopa mon-nieri exercise was statistically significant(P<0.01)at 21 days,the difference between bacopa monnieri exercise and bacopa monnieri non-exercise was statistically significant(P<0.05),and the difference between the control non-exer-cise and bacopa monnieri group at 28 days was statistically significant(P<0.05).The results of the MDA content as-say showed that the difference between the control group and bacopa monnieri exercise was statistically significant(P<0.05).The difference was statistically significant(P<0.05).The SOD vitality value assay showed that the control exercise was statistically significant(P<0.01)compared to the Morinda officinalis group.Conclusion Morinda offi-cinalis can helps the recovery of sciatic nerve injury in mice,and the combination of morinda officinalis and exercise has better recovery effect.