炒制饮片焦栀子中4种多环芳烃测定及初步风险评估

Determination and preliminary risk assessment of 4 polycyclic aromatic hydrocarbons in Gardeniae Fructus Praeparatus

目的建立对炒制饮片焦栀子中4种多环芳烃含量测定方法,并利用单因子污染指数和综合污染指数对其进行风险评估。方法采用高效液相-荧光检测法测定焦栀子中苯并(α)蒽、䓛、苯并(b)荧蒽、苯并(α)芘4种多环芳烃的含量;采用单因子污染指数和综合污染指数对其风险进行评估。结果4种多环芳烃线性关系良好,相关系数r≥0.999,回收率在75.82%~81.83%,RSD≤2.5%。风险评估结果表明焦栀子中苯并(α)蒽、苯并(b)荧蒽和䓛的污染程度相当且较大,远高于苯并(α)芘。同时,综合污染指数>1的占比为69.57%,提示4种多环芳烃污染较为严重,表明焦栀子多环芳烃污染风险需要关注,需进一步加强其风险监测和风险评估。结论该方法可以用于焦栀子中4种多环芳烃的测定及其风险评估,为炒制饮片焦栀子的多环芳烃污染水平进行监测,为其安全风险预警及保障人民用药安全提供数据依据。

Objective To determine the content of 4 polycyclic aromatic hydrocarbons(PAHs)in Gardeniae Fructus Praeparatus,and to assess the risks by single-factor pollution index and comprehensive pollution index.Methods The content of 4 PAHs including benzo(α)anthracene,fen,benzo(b)fluoranthene and benzo(α)pyrene in Gardeniae Fructus Praeparatus was determined by high performance liquid phasefluorescence detection method.Moreover,the risks were assessed with single factor pollution index(Pi)and comprehensive pollution index(Pz).Results The 4 PAHs had a good linear relationship with the correlation coefficient r≥0.999,the recoveries ranged from 75.82%to 81.83%,and the RSDs were all less than 2.5%.The risk assessment showed that the contamination level of benzo(α)anthracene,benzo(b)fluoranthene and fen in Gardeniae Fructus Praeparatus was similar and larger,much higher than that of benzo(α)pyrene.At the same time,the comprehensive pollution index P_(z)>1 accounted for 69.57%,indicating that the 4 PAHs were more seriously polluted.The pollution risk of PAHs in Gardeniae Fructus Praeparatus should be attentioned.Conclusion This method can be used to determine the content and assess the risk of 4 PAHs in Gardeniae Fructus Praeparatus.The level of PAHs should be monitored to ensure the safety of medication use.