卵巢癌患者血清Romo-1、sFRP-4、MRPL15水平及与淋巴结转移及预后生存关系

Correlation between the levels of serum reactive oxygen modulator-1,secreted frizzled-related protein 4 and mitochondrial ribosomal protein L15(MRPL15)of patients with ovarian cancer and their lymph node metastasis and prognosis

目的:探讨卵巢癌(OC)患者血清活性氧调节剂-1(Romo-1)、分泌型卷曲蛋白4(sFRP-4)、线粒体核糖体蛋白L15(MRPL15)水平及与淋巴结转移及预后生存关系。方法:回顾性收集本院2019年7月-2021年3月住院治疗的132例OC患者为OC组,良性病变患者为病变组,体检健康者132例对照组。对OC患者随访3年分为死亡组(n=82)和生存组(n=50)。检测各组血清Romo-1、sFRP-4、MRPL15水平;Cox回归分析影响OC患者预后的相关因素;运用受试者工作特征(ROC)曲线分析血清Romo-1、sFRP-4、MRPL15对OC患者预后生存的预测价值。结果:对照组、病变组、OC组血清Romo-1、MRPL15水平依次升高,血清sFRP-4水平依次降低;OC组淋巴结转移患者血清Romo-1、MRPL15水平高于未转移患者,血清sFRP-4水平低于未转移患者(均P<0.05)。死亡组FIGO分期Ⅲ期~Ⅳ期占比(86.6%)、淋巴结转移占比(74.4%)、低分化程度占比(58.5%)、血清Romo-1(3.81±0.67 ng/ml)、MRPL15(13.26±2.28 ng/ml)水平均高于生存组(48.0%、48.0%、28.0%、2.92±0.58 ng/ml、10.52±1.73 ng/ml),而血清sFRP-4水平(1.75±0.42 ng/ml)低于生存组(2.36±0.49 ng/ml)(均P<0.05)。血清Romo-1、sFRP-4、MRPL15、淋巴结转移、FIGO分期高、分化程度低为影响OC患者预后生存的相关因素(均P<0.05)。血清Romo-1、sFRP-4、MRPL15联合预测OC患者预后生存的曲线下面积为0.957,优于各自单独指标预测(P<0.05)。结论:OC患者血清Romo-1、MRPL15水平异常升高,血清sFRP-4水平异常降低,且与患者淋巴结转移及预后有关,3者联合检测对患者预后生存有较高的预测价值。

Objective:To explore the correlation between the levels of serum reactive oxygen modulator-1(Romo-1),secreted frizzled-related protein 4(sFRP-4)and mitochondrial ribosomal protein L15(MRPL15)of patients with ovarian cancer(OC)and their lymph node metastasis(LNM)and prognosis.Methods:132 women with OC who were hospitalized treatment from July 2019 to March 2021 were collected in group A retrospectively,132 women with benign lesions were collected in group B,and 132 healthy women who underwent physical examinations were collected in group C.Based on the results of the women within 3 years of follow-up,the women in group A were divided into group A1(82 women with death)and group A2(50 women with survival).The levels of serum Romo-1,sFRP-4,and MRPL15of the women in these groups were detected.Cox regression was applied to analyze the relevant factors affecting the prognosis of the women with OC.Receiver operating characteristic(ROC)curve was applied to analyze the values of the levels of serum Romo-1,sFRP-4,and MRPL15 of the women with OC for predicting their prognosis.Results:The levels of serum Romo-1 and MRPL15 of the women in group C,in group B and in group A had increased gradually,and the serum sFRP-4 level of the women in group C,in group B and in group A had decreased gradually.In group A,the levels of serum Romo-1 and MRPL15 of the women with LNM were significantly higher than those of the women without LNM,and the serum sFRP-4 level of the women with LNM was significantly lower than that of the women without LNM(all P<0.05).The proportions of the OC FIGO stageⅢ-Ⅳ(86.6%),the LNM(74.4%),the poor differentiation of OC cells(58.5%),the serum Romo-1 level(3.81±0.67 ng/ml)and the serum MRPL15 level( 13.26±2.28 ng/ml)of the women in group A1 were significantly higher than those(48.9%,48.0%,28.0%,2.92±0.58 ng/ml and 10.52±ng/ml)of the women in group A2(P<0.05).The serum sFRP-4 level(1.75±0.42 ng/ml)of the women in group A1 was significantly lower than that(2.36±0.49 ng/ml)of the women in group A2(all P<0.05).The levels of serum Romo-1,sFRP-4 and MRPL15,the LNM,the high FIGO stage and the low differentiation of OC cells of the women with OC were all the related factors affecting their prognosis and survival(all P<0.05).The area under the curve of the combined the levels of serum Romo-1,sFRP-4 and MRPL15 of the women with OC for predicting their prognosis and survival was 0.957,and which was significantly superior to that of the serum Romo-1 level,the serum sFRP-4 level or the serum MRPL15 level alone(P<0.05).Conclusion:The levels of serum Romo-1 and MRPL15of the women with OC are obviously increased,and the serum sFRP-4 level of the women with OC is obviously decreased,and all of which are closely related to the LNM and the prognosis of the women.The combined detections of the levels of serum Romo-1,MRPL15 and sFRP-4 of the women with OC have higher predictive values for their prognosis.