基于相位恢复的透明样本3维重建系统

Three-dimensional reconstruction system for transparent samples based on phase retrieval

为解决传统显微成像技术难以获取无色透明样本结构和厚度的问题,设计了一款小型透明样本3维重建系统。该系统通过对透明样本进行相位恢复,实现3维重建。系统的设计突破了传统光学结构,只需输入携带样本信息的光线,经过分光棱镜分成两路,终由双目相机捕获。系统使用3D打印制作,尺寸仅为110 mm×110 mm×60 mm,成本低廉,并可与传统显微成像设备配合使用。系统内置自动对焦和视场配准算法,只需采集1张过焦和1张欠焦图像,通过求解光强传输方程便可进行相位恢复,从而实现透明样本的3维重建。测试结果显示,10倍物镜下系统的成像分辨率可达2.46μm,同时相位恢复精确度也能达到基本要求。系统成功对血细胞和载玻片划痕进行了3维重建,证明了系统的可行性与实用性。

In response to the difficulties posed by traditional microscopy imaging techniques in capturing the structure and thickness of colorless transparent samples,we have designed a miniature three-dimensional reconstruction system for such samples.This innovative system,breaking away from traditional optical structures,performs phase retrieval on transparent samples to achieve three-dimensional reconstruction.It requires only light carrying sample information,which is then bifurcated by a spectroscope and captured by a stereo camera.Constructed using 3D printing technology,the compact system measures just 110 mm×110 mm×60 mm,offering a cost-effective solution that is also compatible with traditional microscopy imaging equipment.It incorporates autofocus and field of view correction algorithms,which,by collecting one over-focused and one under-focused image,solve the transport intensity equation to enable phase retrieval and hence the three-dimensional reconstruction of transparent samples.Test results have shown that the system can achieve an imaging resolution of 2.46μm under a 10×objective lens,and the phase recovery accuracy can also meet the basic requirements.Furthermore,the successful three-dimensional reconstruction of blood cells and scratches on microscope slides validates the system's feasibility and practicality.