金线莲多糖及其衍生物抗氧化活性及抗疲劳研究

Study on Antioxidant and Antifatigue Activities of Polysaccharides from Anoectochilus roxburghii and the Derivatives

通过对金线莲多糖进行硫酸化、磷酸化和羧甲基化修饰,得到其多糖衍生物,利用1,1-二苯基-2-三硝基苯肼(DPPH)自由基、亚硝酸盐和一氧化氮(NO)的清除模型比较其抗氧化活性,并采用小鼠负重游泳试验比较其抗疲劳能力,以确定金线莲多糖的最优衍生方法。结果表明:修饰后金线莲多糖的分子量和蛋白质含量均有所降低。金线莲多糖衍生物和金线莲多糖的190~800 nm全波长吸收光谱具有一定的相似性。傅里叶变换红外光谱证实-OH分别转化形成了O-S、-COO和P-OH键。硫酸化金线莲多糖具有较强的DPPH自由基、亚硝酸盐和NO的清除率。硫酸化金线莲多糖具有最强的抗疲劳能力,可通过降低血清中的肌酸激酶(CK)和尿素氮(BUN)含量,提高乳酸脱氢酶(LDH)活性及Na^(+)-K^(+)-ATP酶、Ca^(2+)-Mg^(2+)-ATP酶活性和三磷腺苷(ATP)含量,改善机体的能力代谢,以达到抗疲劳的目的。硫酸化是一种较优的金线莲多糖的衍生方法。

The polysaccharide derivatives were obtained by sulfation,phosphorylation and carboxymethylation modification of the Anoectochilus roxburghii polysaccharides.The antioxidant activities were compared by scavenging model of 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)free radical,nitrite and nitric oxide(NO),and the antifatigue abilities were compared by weight-bearing swimming test in mice,in order to determine the optimal method for the derivation of A.roxburghii polysaccharides.The results showed that the molecular weights and protein contents of the modified polysaccharides were lower than those of A.roxburghii polysaccharides,while the 190~800 nm full-wavelength absorption spectra presented a certain similarity.Fourier transform infrared spectroscopy confirmed that the-OH groups were transformed into O-S,-COO,and P-OH bonds,respectively.Sulfated A.roxburghii polysaccharides had strong scavenging effects on DPPH free radicals,nitrite,NO,and the strongest antifatigue ability,which could improve the ability metabolism of the body by reducing the contents of creatine kinase(CK)and urea nitrogen(BUN)in sera,increasing the activities of lactate dehydrogenase(LDH),and improving the activities of Na^(+)-K^(+)-ATPase,Ca^(2+)-Mg^(2+)-ATPase,and ATP contents to achieve the goal of antifatigue.Therefore,sulfation would be a superior method for the derivation of polysaccharides from A.roxburghii.