乳腺癌类器官的培养及鉴定

Culture and characterization of breast cancer organoids

目的建立乳腺癌类器官并长期培养,鉴定其相关分子特征及对相关药物的敏感性。方法乳腺癌患者手术标本,胶原酶消化法,获取细胞进行类器官培养,进行形态学观察,免疫荧光检测ER、PR、HER-2、CK表达,对其中3例类器官进行紫杉醇、多柔比星、顺铂药敏测试。结果共培养建立了44例乳腺癌类器官,均呈实性类球状生长。类器官ER、PR、HER-2、CK表达情况与患者临床样品一致。乳腺癌类器官对化疗药紫杉醇、多柔比星、顺铂具有一定敏感性。结论乳腺癌类器官作为新型体外模型,具有一定异质性,再现体内病生理功能特性。

Objective To establish breast cancer organoids for a long time and to characterize their molecular expression and test their sensitivity to chemotherapy drugs.Methods Breast cancer specimens were digested with collagenase to release cancer cells for organoid culture.The organoids were characterized by morphology and ER,PR,HER-2,CK expression by technology of histoimmunofluorescence.Among them,three were tested for paclitaxel,doxorubicin and cisplatin sensitivity.Results A total of 44 cases of breast cancer organoids were established,all of which showed dense spherical-like growth and ER,PR,HER-2,CK,matching their clinical counterpart.Breast cancer organoids were sensitive to chemotherapy drugs paclitaxel,doxorubicin and cisplatin.Conclusions Organoid model,as a new in vitro may reproduce the pathophysiology features with heterogeneity.