6个铁线莲品种叶片解剖结构与植株耐热性的比较

Comparison of Leaf Anatomy and Plant Heat Tolerance in Six Clematis Cultivars

为明确不同铁线莲品种的叶片结构与其耐热性的关系,本研究利用扫描电镜观察了6个市场热销的铁线莲品种叶片结构,并对叶片厚度、表皮细胞、栅栏组织厚度等15项指标进行聚类分析和相关性分析,筛选出最具代表性的耐热性鉴定指标,利用平均隶属函数分析6个铁线莲品种耐热性的综合评价。结果表明:6个铁线莲品种叶片解剖结构显示上表皮厚度普遍大于下表皮厚度,栅栏组织细胞排列紧密,胞间隙小,海绵组织排列疏松,细胞间隙大而多,大多数海绵组织厚度大于栅栏组织厚度。对栅栏组织厚度等5个代表性指标进行隶属函数分析,得到6个铁线莲品种的耐热性排序为‘啤酒’>‘朱莉’>‘蓝光’>‘紫铃铛’>‘紫色旋律’>‘前卫’。该评价方法为更科学有效地评价铁线莲耐热性提供了参考价值,为筛选耐热种质和开展育种研究奠定基础。

In order to clarify the relationship between the leaf structure of different Clematis varieties and their heat resistance,this study used scanning electron microscopy to observe the leaf structure of six marketable Clematis varieties,and carried out cluster analysis and correlation analysis on 15 indexes such as leaf thickness,epidermal cells,and thickness of palisade tissues,screened out the most representative indexes for heat resistance identification,and analyzed the comprehensive evaluation for heat resistance of six Clematis varieties by using the average affiliation function.The results showed that the anatomical structure of the leaves of six Clematis varieties showed that the thickness of upper epidermis was generally greater than that of the lower epidermis,the cells of the palisade tissues were closely arranged,with small cell gaps,and the spongy tissues were loosely arranged,with large and numerous cell gaps,and most of the spongy tissues were greater than the thickness of the palisade tissues.The affiliation function analysis of five representative indexes,such as the thickness of palisade tissue,obtained that the heat resistance of six Clematis varieties was ranked as BiJou'Evipo030'>'Julie'>'Blue light'>'Purple bell'>'Purple melody'>'Avant'.This evaluation method provides a reference value for a more scientific and effective evaluation of the heat resistance of Clematis,and lays a foundation for screening heat-resistant germplasm and carrying out breeding research.