基于高通量测序技术的朝鲜淫羊藿内生真菌多样性分析

Diversity Analysis of Endophytic Fungi in Epimedium koreanum Based on High-throughput Sequencing

本研究采用llumina Mi Seq高通量测序技术结合高效液相色谱等方法,对朝鲜淫羊藿叶内生真菌群落多样性与药效成分进行分析,探寻二者相关性及其优势菌属。实验结果表明朝鲜淫羊藿叶中共存有686种真菌,归属于7门、32纲、97目、242科、451属,其中子囊菌门(Ascomycota)、担子菌门(Basidiomycota)占比分别为99.05%、99.22%;圆孢多臂菌属和短柄霉属为叶中核心菌属;在相对丰度高于1%的菌属中,横断孢属、念珠菌属、线黑粉酵母属、隐球菌属与黄酮类化学成分呈正相关,杯梗孢属、节枝孢属、外瓶柄霉属、炭疽菌属与黄酮类化学成分呈负相关。该研究为朝鲜淫羊藿质量提升提供科学依据。

In this study,the diversity of endophytic fungi in Epimedium koreanum leaves and their effective components were analyzed by high-throughput sequencing and high performance liquid chromatography(HPLC)to explore the correlation between them and their dominant genera.The results showed that there were 686 species of fungi in the leaves of E.koreanum,belonging to 7 phyla,32 classes,97 orders,242 families and 451 genera,among which Ascomycota and Basidiomycota accounted for 99.05%and 99.22%respectively;Trichomerium and Aureobasidium are the core genera in leaves;among the genera with relative abundance higher than 1%,there was a positive correlation between the flavonoid chemical components and the genera of Strelitziana,Candida,Filobasidium and Cryptococcus,and a negative correlation between the flavonoid chemical components and the genera of Cyphellophora,Articulospora,Exophiala and Colletotrichum.This study provides a scientific basis for the quality improvement of E.koreanum.