三阴性乳腺癌中SOX10表达的临床意义及与上皮间质转化的相关性

Clinical significance of SOX10 expression in triple negative breast cancer and correlation with EMT

目的探讨Y框蛋白10基因(SRY‑related hmg‑box 10,SOX10)在三阴性乳腺癌(triple negative breast cancer,TNBC)组织中表达的临床意义及其与上皮间质转化(epithelial‑mesenchymal transition,EMT)的相关性。方法采用免疫组织化学方法检测80例TNBC及60例非TNBC组织SOX10的表达水平,同时检测TNBC组织中EMT关键标志物波形蛋白(vimentin)和上皮钙黏素(E‑cadherin)的表达水平,分析TNBC中SOX10、vimentin和E‑cadherin的表达与患者临床病理参数之间的关系及SOX10与EMT标志物的相关性。结果SOX10在TNBC中的表达明显高于非TNBC,差异有统计学意义(P<0.001)。SOX10的表达与TNBC患者的肿瘤分型、组织学分级、Ki67高增殖表达(Ki67≥30%)及淋巴结转移相关(P<0.05),与年龄、肿瘤最长径无关。SOX10的表达与vimentin表达呈正相关(r=0.398,P<0.001),与E‑cadherin表达呈负相关(r=-0.337,P<0.01)。结论SOX10在TNBC中特异性高表达,可作为TNBC鉴别诊断及预后的有效生物学标志物。SOX10可能通过调控EMT相关蛋白的表达促进肿瘤的侵袭和转移。

Objective:To investigate the clinical significance of SOX10 expression in triple negative breast cancer(TNBC)tissues and its correlation with epithelial‑mesenchymal transition(EMT).Methods:To use Immunohistochemical methods to detect the expression levels of SOX10 protein in 80 cases of TNBC and 60 cases of non TNBC tissues,as well as the expression levels of EMT key markers Vimentin and E-cadherin in TNBC tissues and to analyze the relationship between SOX10,Vimentin and E-cadherin in TNBC and clinicopathological parameters and the correlation between SOX10 and EMT markers.Results:SOX10 expression was significantly higher in TNBC than that in non-TNBC(P<0.001).The expression of SOX10 was associated with tumor classification,histological grade,Ki67 high value-added expression(Ki67≥30%)and lymph node metastasis in TNBC patients(P<0.05),and has no relationship with age or tumor diameter.The expression of SOX10 was positively correlated with Vimentin expression(r=0.398,P<0.001)and negatively correlated with E-cadherin expression(r=-0.337,P<0.01).Conclusion:SOX10 is specifically and highly expressed in TNBC and can be used as a useful biological marker for the differential diagnosis and prognosis of TNBC.SOX10 may promote tumor invasion and metastasis by regulating the expression of EMT-related proteins.