苗药联合揿针对原发性失眠模型大鼠血清IL-1、TNF-α含量的影响

Effect of Combined Press-needle of Miao Medicine on Serum IL-1 and TNF-αLevels in Rats with Primary Insomnia Model

目的:探究苗药联合揿针对原发性失眠模型大鼠血清IL-1、TNF-α含量的影响。方法:采用对氯苯丙氨酸诱导构建原发性失眠大鼠模型,分为空白组、模型组、苗药组、揿针组、苗药联合揿针组。通过干预治疗后,观察大鼠一般状态、记录治疗前后大鼠体重差值,通过Elisa法检测血清IL-1、TNF-α水平。结果:与空白组相比,PI模型大鼠一般状态较差,体重增加较少(P<0.01),血清IL-1、TNF-α水平明显增高(P<0.01);与模型组相比,苗药组、揿针组体重增加较多(P<0.05,P<0.05),血清IL-1、TNF-α水平下降(P<0.05),苗药联合揿针组体重增长趋势更好(P<0.05),血清IL-1、TNF-α水平明显下降(P<0.01),接近于空白组水平。结论:苗药验方内服、揿针埋穴、苗药验方内服联合揿针埋穴均能改善睡眠症状,且二者联合治疗效果呈现更好的趋势。通过影响原发性失眠模型大鼠血清IL-1、TNF-α的水平可能是对失眠发挥治疗作用的机制之一。

Objective To explore the effects of Miao combined with snappers on serum IL-1 and TNF-αlevels in rats with primary insomnia model.Methods A rat model of primary insomnia was constructed using p-chlorophenylalanine induction.The rats were divided into blank group,model group,Miao medicine group,press-needle group and Miao medicine combined press-needle group.After the intervention,the general state of the rats was observed,the difference in body weight before and after the treatment was recorded,and the serum IL-1 and TNF-αlevels were detected by Elisa method.ResultsCompared with the blank group,PI model rats had poorer general status,less weight gain(P<0.01),and significantly higher serum IL-1 and TNF-αlevels(P<0.01);compared with the model group,the Miao medicine group and the press-needle group had more weight gain(P<0.05,P<0.05),and decreased serum IL-1 and TNF-αlevels(P<0.05),and the Miao medicine combined with the press-needle group showed a better trend of weight gain(P<0.05),and serum IL-1 and TNF-αlevels decreased significantly(P<0.01),close to the level of the blank group.Conclusion Internal administration of Miao medicinal formula,press-needle burying points,and internal administration of Miao medicinal formula combined with press-needle burying points could improve sleep symptoms,and the combined treatment effect of the two showed a better trend.By affecting the levels of serum IL-1 and TNF-αin rats with primary insomnia model,it may be one of the mechanisms to exert therapeutic effects on insomnia.