红绶曲霉ACB1042与四种酰胺类杀虫剂的相容性及其对亚洲玉米螟的室内联合毒力

Compatibility of Aspergillus nomius ACB1042 with four amide insecticides and their indoor joint toxicity to Ostrinia furnacalis(Lepidoptera:Pyralidae)

【目的】明确红绶曲霉Aspergillus nomius ACB1042与4种酰胺类杀虫剂的相容性及混配后对亚洲玉米螟Ostrinia furnacalis的毒力。【方法】以4种酰胺类杀虫剂20%氯虫苯甲酰胺SC(悬浮剂)、10%溴氰虫酰胺OF(油悬剂)、10%四氯虫酰胺SC和20%四唑虫酰胺SC的田间推荐浓度为初始浓度,采用血球计数法和十字交叉法分别测定这4种杀虫剂对红绶曲霉ACB1042孢子萌发抑制率、菌丝生长抑制率和产孢抑制率的影响;通过喷雾法测定红绶曲霉ACB1042和20%四唑虫酰胺SC对亚洲玉米螟3龄幼虫的致死中浓度(median lethal concentration,LC_(50))值以及LC_(50)的红绶曲霉ACB1042与LC_(50)的20%四唑虫酰胺SC以体积比9∶1,4∶1,1∶1,1∶4,1∶9混配处理对亚洲玉米螟3龄幼虫的室内联合毒力,测定LC_(50)的20%四唑虫酰胺SC与LC_(50)的红绶曲霉ACB1042以体积比4∶1混剂处理玉米螟3龄幼虫后于24,48,72,96和120 h体内过氧化物酶(peroxidase,POD)、超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)以及多酚氧化酶(polyphenol oxidase,PPO)、羧酸酯酶(carboxylesterase,CarE)和谷胱甘肽S-转移酶(glutathione S-transferase,GST)活性。【结果】4种杀虫剂在田间推荐浓度下对红绶曲霉ACB1042的孢子萌发率、菌丝生长速率和产孢量均有一定的抑制作用,随着杀虫剂稀释倍数增加,抑制作用减弱,其中20%四唑虫酰胺SC 10倍稀释浓度(0.1 RC)与红绶曲霉ACB1042具有较好的相容性:红绶曲霉ACB1042孢子萌发抑制率、产孢抑制率和9 d菌丝生长抑制率依次为0,2.15%和0.78%。红绶曲霉ACB1042与20%四唑虫酰胺SC以不同体积比混配后的共毒系数(co-toxicity coefficient,CTC)均大于120,其中20%四唑虫酰胺SC与红绶曲霉ACB1042以体积比4∶1混用后协同增效作用最好,对亚洲玉米螟3龄幼虫的LC_(50)值为2.359 mg/L,CTC高达365;红绶曲霉ACB1042与20%四唑虫酰胺SC混剂处理后亚洲玉米螟3龄幼虫体内POD,SOD,CAT,PPO,CarE和GST活性随着处理时间推移均呈先上升后下降的趋势。【结论】在田间防治亚洲玉米螟时,可利用红绶曲霉ACB1042与低浓度的20%四唑虫酰胺SC混配,从而达到增效作用。

【Aim】To clarify the compatibility of Aspergillus nomius ACB1042 with four amide insecticides and the joint toxicity to Ostrinia furnacalis after mixing.【Methods】The recommended field concentrations of four amide insecticides,20%chlorantraniliprole suspension concentrate(SC),10%cyantraniliprole oilmiscible flowable concentrate(OF),10%tetrachlorantraniliprole SC and 20%tetrazolfenamide SC,were used as the initial concentrations,and the effects of the four insecticides on the spore germination inhibition rate,inhibition rate of mycelial growth and sporulation inhibition rate of A.nomius ACB1042 were measured by hemocyte counting and crossover methods,respectively.The median lethal concentration(LC_(50))values of A.nomius ACB1042 and 20%tetrazolfenamide SC against the 3rd instar larvae of O.furnacalis by spraying method as well as the indoor joint toxicity of a mixture of LC_(50) of A.nomius ACB1042 and LC_(50) of 20%tetrazolfenamide SC treatments in the volume ratios of 9∶1,4∶1,1∶1,1∶4 and 1∶9 against the 3rd instar larvae of O.furnacalis,and the activities of peroxidase(POD),superoxide dismutase(SOD),catalase(CAT),polyphenol oxidase(PPO),carboxylesterase(CarE)and glutathione S-transferase(GST)in the 3rd instar larvae of O.furnacalis treated with LC_(50) of 20%tetrazolfenamide SC and LC_(50) of A.nomius ACB1042 in the mixture of 4∶1 volume ratio at 24,48,72,96 and 120 h after treatment were determined.【Results】The four insecticides had certain inhibitory effects on the spore germination rate,mycelial growth rate and sporulation of A.nomius ACB1042 at the recommended field concentration,and the inhibitory effects were weakened with the increase of insecticide dilution fold,among which,20%tetrazolfenamide SC 10-fold dilution concentration(0.1 RC)had a better compatibility with A.nomius ACB1042,with the germination inhibition rate,the sporulation inhibition rate,and the inhibition rate of mycelial growth for 9 d of 0,2.15%and 0.78%,respectively.The co-toxicity coefficients(CTCs)of A.nomius ACB1042 and 20%tetrazolfenamide SC in different volume ratios were all greater than 120,and the mixture of 20%tetrazolfenamide SC and A.nomius ACB1042 in the volume ratio of 4∶1 had the best synergistic effect,with the LC_(50) value of 2.359 mg/L against the 3rd instar larvae of O.furnacalis,and the CTC was as high as 365.The activities of POD,SOD,CAT,PPO,CarE and GST in the 3rd instar larvae of O.furnacalis after treatment with the mixtures of A.nomius ACB1042 and 20%tetrazolfenamide SC showed a trend of increasing and then decreasing.【Conclusion】In field control of O.furnacalis,A.nomius ACB1042 can be mixed with 20%tetrazolfenamide SC at low concentrations to achieve synergic effect.