抗菌丁香酚/介孔二氧化硅纳米颗粒的制备及其医用潜力评价

Preparation of antibacterial eugenol/mesoporous silica nanoparticles and their medical potential evaluation

为了解决天然抗菌丁香酚(EG)应用过程中易挥发、利用率低的问题,采用St9ber法制备高比表面积、微孔径可控、热稳定性和生物兼容性优的丁香酚专用介孔二氧化硅微粒(MSNs),探究其在敷料应用场景下的长效缓释、高效抗菌等性能。设计制备的MSNs粒径范围为100~200 nm,孔径6.54 nm,比表面积998.05 m^(2)/g,丁香酚固载量达57.09%。实验证明丁香酚/介孔二氧化硅纳米颗粒(EG@MSNs)具有快速抗菌效果,而EG@MSNs负载的非织材料基海藻酸钙敷料(EG@MSNs/CA@NWs)经过恒温培养箱培养15 d后,对大肠杆菌仍保持良好的抑菌效果,且该抗菌敷料的凝血时间缩短71.40%。此外,EG@MSNs/CA@NWs敷料的荧光物、表面活性物、水中溶出物和水浸出液酸碱度均表现优异。文章设计制备的EG@MSNs有望在伤口、创面抗菌修复领域展现良好的实际应用潜力。

Eugenol(EG)is an important bioactive compound extracted from natural clove.There are 9.4-14.7 g eugenol essential oil contained in every 100g fresh clove.Eugenol has various health benefits,including anti-cancer,antioxidant,preservative,and anti-depressant properties.It also demonstrates broad-spectrum antibacterial activity effective against a wide range of bacteria and fungi,making it a highly desirable controlled and natural antibacterial material for wound care applications such as wound healing and hemostasis.However,due to the rapid volatility and chemical instability of eugenol essential oil,its utilization rate is low in practical applications,often requiring encapsulation for use.To address the issues of easy volatility and low utilization efficiency of eugenol in practical applications,mesoporous silica nanoparticles(MSNs)are introduced.MSNs have been widely studied in the fields of environment,energy,and biotechnology due to their high specific surface area,tunable pore size,proness to functionalization,as well as excellent thermal stability,chemical stability,and biocompatibility.It is expected that these characteristics can be utilized to achieve efficient and sustained release of eugenol.In this paper,an improved St ber method was used to prepare MSNs with a high specific surface area for immobilizing the natural antibacterial active substance EG,to synthesize aromatic antibacterial nanoparticles(EG@MSNs)with excellent thermal stability,high eugenol loading capacity,and rapid dissolution rate.The prepared eugenol-specific mesoporous silica particles had a particle size range of 100-200 nm,a pore size of 6.54 nm,and a specific surface area of 998.05 m^(2)/g.The test results showed that the eugenol loading capacity reached 57.09%.The rapid antibacterial effect of EG@MSNs was verified through testing in simulated wound tissue fluid,where the instantaneous dissolution rate at a fitted dispersal concentration of 2.35 mg/mL for EG@MSNs can reach a level comparable to that achieved by adding an excessive amount of EG.This allows EG@MSNs to maximize their effectiveness in wound dressings for trauma treatment and has the potential to synergize with"Moist Wound Healing"theory,quickly creating an antibacterial liquid microenvironment.Furthermore,the EG@MSNs were uniformly loaded onto nonwoven calcium alginate dressings(CA@NWs)by using the spray atomization technique resulting in a dressing with over 15 days of inhibition against Escherichia coli and a 71.4%reduction in clotting time.The dressing also exhibited excellent performance in terms of fluorescent substances and surface-active agents,water soluble substances,and pH of water immersion extract.The research results demonstrate that eugenol/mesoporous silica nanoparticles have a rapid antibacterial effect and show great potential for practical applications in the field of wound antibacterial repair.