不同时期RMPP患儿支气管肺泡灌洗液SPA表达及与肺功能的关系

Expression of SPA in Bronchoalveolar Lavage Fluid of Children With RMPP at Different Periods and Its Relationship With Lung Function

目的探讨不同时期难治性支原体肺炎(refractory mycoplasma pneumoniae pneumonia,RMPP)患儿支气管肺泡灌洗液中表面活性物质相关蛋白-A(surfactant protein A,SPA)的表达水平及与肺功能的关系。方法选取2021年1月—2023年1月南通市第一人民医院收治的62例RMPP患儿为对象,以同期行支气管镜异物取出术且无肺部感染的50例患儿为对照组。RMPP患儿于急性期、恢复期行支气管肺泡灌洗液中SPA检测以及肺功能指标检测[第1秒用力呼气容积(forced expiratory volume in one second,FEV_(1))、用力肺活量(forced vital capacity,FVC)、FEV_(1)/FVC],对照组同样如此。比较三者检测结果差异,使用Pearson分析RMPP患儿支气管肺泡灌洗液中SPA水平与肺功能指标的相关性。结果RMPP患儿肺泡灌洗液中的SPA急性期(59.82±12.64)μg/L、恢复期(129.91±24.86)μg/L高于对照组,差异有统计学意义(P<0.05)。RMPP急性期、RMPP恢复期的FEV_(1)、FVC、FEV_(1)/FVC(80.95±6.88)%、(88.41±5.75)%较对照组低,差异有统计学意义(P<0.05);RMPP患儿于急性期时肺泡灌洗液中的SPA较恢复期低,FEV_(1)、FVC、FEV_(1)/FVC较恢复期低,差异有统计学意义(P<0.05);RMPP患儿肺泡灌洗液中SPA水平与FEV_(1)、FVC、FEV_(1)/FVC呈正相关(P<0.05)。结论RMPP患儿病情处于急性发展阶段的情况下其支气管肺泡灌洗液中的SPA表达相较病情恢复阶段更低,同时RMPP患儿支气管肺泡灌洗液中SPA水平与其肺功能指标呈正相关性。

Objective To explore the expression levels of surfactant associated protein-A(SPA)in bronchoalveolar lavage fluid of children with refractory mycoplasma pneumonia(RMPP)at different stages and its relationship with lung function.Methods A total of 62 children with RMPP admitted to the Nantong First People's Hospital from January 2021 to January 2023 were selected as the subjects,and 50 children who underwent bronchoscopic foreign body removal surgery during the same period without pulmonary infection were selected as the control group.During the acute and recovery phases,children with RMPP underwent SPA detection in bronchoalveolar lavage fluid,as well as lung function indicators such as forced expiratory volume(FEV_(1)),forced vital capacity(FVC),and FEV_(1)/FVC.The same was true for the control group.The differences in the results of the three tests and use Pearson analysis to examine the correlation between SPA levels in bronchoalveolar lavage fluid and lung function indicators in children with RMPP were compared.Results The SPA in alveolar lavage fluid of children with RMPP was(59.82±12.64)μg/L in the acute phase and(129.91±24.86)μg/L in the recovery phase was higher than that of the control group,the differences were statistically significant(P<0.05).The FEV_(1),FVC and FEV_(1)/FVC during the acute phase and convalescence were lower than those in the control group,the requirement for statistical significance was met(P<0.05).During the acute phase,the SPA in the bronchoalveolar lavage fluid of RMPP patients was lower than that in the recovery phase,and FEV_(1),FVC,and FEV_(1)/FVC(80.95±6.88)%、(88.41±5.75)%were lower than those in the recovery phase,with statistically significant differences(P<0.05).The level of SPA in bronchoalveolar lavage fluid of children with RMPP was positively correlated with FEV_(1),FVC and FEV_(1)/FVC(P<0.05).Conclusion Children with acute RMPP SPA in bronchoalveolar lavage fluid are lower than those of recovery,At the same time,the levels of SPA in the bronchoalveolar lavage fluid of children with RMPP are positively correlated with their lung function indicators.