胃腺癌组织拓扑异构酶Ⅱα、周期蛋白G1表达与临床病理特征、幽门螺杆菌感染的关系研究

Expression of topoisomeraseⅡαand cyclin G1 in gastric adenocarcinoma and its relationship with clinicopathological features and Helicobacter pylori infection

目的分析胃腺癌组织拓扑异构酶Ⅱα(TopoⅡα)、细胞周期蛋白G1(cyclin G1)表达及其与临床病理特征、幽门螺杆菌(Hp)感染的关系。方法回顾性收集2018年5月至2022年5月在六安市中医院就诊的217例胃腺癌患者的癌组织及癌旁正常组织标本,采用免疫组织化学染色(S-P)法检测TopoⅡα、cyclin G1表达。比较胃腺癌组织及癌旁正常组织TopoⅡα、cyclin G1表达的差异;采用单因素及多因素Logistic回归分析胃腺癌组织TopoⅡα、cyclin G1表达与临床病理特征的关系;比较不同TopoⅡα、cyclin G1表达胃腺癌患者Hp感染情况,分析TopoⅡα、cyclin G1表达与Hp感染的相关性。结果胃腺癌组织TopoⅡα、cyclin G1阳性表达率均高于癌旁组织[60.83%(132/217)比29.95%(65/217)、62.67%(136/217)比0],差异有统计学意义(χ^(2)=41.73、198.07,P<0.01)。不同组织学分化程度、T分期、N分期、TNM分期的胃腺癌组织TopoⅡα、cyclin G1阳性表达率比较差异均有统计学意义(P<0.05),且伴远处转移的胃腺癌组织TopoⅡα阳性表达率高于无远处转移的胃腺癌组织(P<0.05)。多因素Logistic回归分析结果显示,组织学分化程度、T分期、N分期是TopoⅡα阳性表达的危险因素(P<0.05);组织学分化程度、T分期、N分期、TNM分期是cyclin G1阳性表达的危险因素(P<0.05)。217例胃腺癌患者中,Hp阳性率为81.57%(177/217);TopoⅡα、cyclin G1阳性表达的胃腺癌患者Hp阳性率高于TopoⅡα、cyclin G1阴性表达患者[87.12%(115/132)比72.94%(62/85)、87.50%(119/136)比71.60%(58/81)],差异有统计学意义(χ^(2)=6.92、8.53,P<0.05)。Kendall tau-b相关系数分析结果显示,TopoⅡα、cyclin G1表达与Hp感染呈正相关(r=0.179、0.198,P<0.05)。结论TopoⅡα、cyclin G1蛋白在胃腺癌组织中有较高的阳性率,并与胃腺癌组织学分化程度、T分期、N分期等病理特征和Hp感染相关。

Objective:To analyze the expression of topoisomeraseⅡα(TopoⅡα)and cyclin G1 in gastric adenocarcinoma and their relationship with clinicopathological characteristics and Helicobacter pylori(Hp)infection.Methods:From May 2018 to May 2022,217 gastric adenocarcinoma tissues and adjacent normal tissue samples were collected in Lu′an Hospital of Traditional Chinese Medicine.The streptavidin-peroxidase method(S-P)was used to detect the expression of TopoⅡαand cyclin G1.The expression of TopoⅡαand cyclin G1 in gastric adenocarcinoma tissues and adjacent normal tissues were compared.The relationship between TopoⅡαand cyclin G1 expression in gastric adenocarcinoma tissues and clinicopathological features were analyzed by univariate and multivariate Logistic regression.In addition,the Hp infection of gastric adenocarcinoma patients with different expressions of TopoⅡαand cyclin G1 was compared.Kendall tau-b correlation coefficient was used to analyze the correlation between TopoⅡαexpression,cyclin G1 expression and Hp infection.Results:The positive rates of TopoⅡαand cyclin G1 in gastric adenocarcinoma were higher than those in adjacent tissues:60.83%(132/217)vs.29.95%(65/217),62.67%(136/217)vs.0,there were statistical differences(χ^(2)=41.73,198.07,P<0.01).There were significant differences in the positive expression rates of TopoⅡαand cyclin G1 in gastric adenocarcinoma patients with different histological differentiation degrees,T stage,N stage and TNM stage(P<0.05).The positive expression rate of TopoⅡαin gastric adenocarcinoma tissues with distant metastasis was significantly higher than that in gastric adenocarcinoma tissues without distant metastasis(P<0.05).Multivariate Logistic regression analysis showed that histological differentiation degree,T stage,histological differentiation degree and N stage were the influencing factors of TopoⅡαexpression(P<0.05);histological differentiation degree,T stage,N stage,TNM stage were the influencing factor of cyclin G1 expression(P<0.05).The positive rate of Hp in 217 patients with gastric adenocarcinoma was 81.57%(177/217).The positive rates of Hp in gastric adenocarcinoma patients with positive TopoⅡαand positive cyclin G1 were significantly higher than those with negative TopoⅡαand cyclin negative G1 expression:87.12%(115/132)vs.72.94%(62/85),87.50%(119/136)vs.71.60%(58/81),there were statistical differences(χ^(2)=6.92,8.53,P<0.05).Kendall tau-b correlation coefficient analysis showed that the expression of TopoⅡαand cyclin G1 were positively correlated with Hp infection(r=0.179,0.198,P<0.05).Conclusions:The positive rate of TopoⅡαand cyclin G1 in gastric adenocarcinoma is high,and it is correlated with histological differentiation,T stage,N stage and Hp infection.