不同益生元体外模拟消化及对7种菌混合培养的研究

Study on simulated in vitro digestion of different prebiotice and mixed culture of 7 kinds of bacteria

研究了6种不同益生元的体外消化特性及其消化后对人体肠道内5种常见有益菌及2种有害菌生长繁殖的影响。选取低聚果糖、低聚半乳糖、低聚木糖、低聚异麦芽糖、水苏糖和抗性糊精这6种益生元,依次模拟口腔消化、胃消化以及小肠消化,对比其消化前后总糖、还原糖及组分分解情况。使用消化后的糖液对5种肠道有益菌以及2种有害菌进行体外培养,评价其对菌群的生长繁殖影响,并分析培养后短链脂肪酸的变化。通过实验可知,6种益生元在模拟体外消化后,组分几乎没有降解,均具有抗消化性;6种益生元的体外模拟消化液对人体肠道有益菌具有不同程度的增殖作用,同时可抑制有害菌的生长繁殖,增加短链脂肪酸含量。

The in vitro digestive characteristics of 6 kinds of prebiotics and their effects on the growth and reproduction of 5 kinds of common beneficial bacteria and 2 kinds of harmful bacteria in human gut were studied.Six prebiotics,fructooligosaccharides,galactooligosaccharides,xylose oligosaccharides,isomaltose oligosaccharides,stachyose and resistant dextrins,were selected to simulate oral digestion,gastric digestion and intestinal digestion,and compare the decomposition of total sugars,reducing sugars and components before and after digestion.Five kinds of intestinal beneficial bacteria and two kinds of harmful bacteria were cultured in vitro with the digested sugar solution,and their effects on the growth and reproduction of the bacteria were evaluated,and the changes of shortchain fatty acids after culture were analyzed.The results showed that after simulated digestion in vitro,the components of the 6 prebiotics were almost not degraded,and all of them had anti-digestibility.The simulated digestive solution of 6 kinds of prebiotics in vitro has different degrees of proliferation of beneficial bacteria in human intestinal tract,and can inhibit the growth and reproduction of harmful bacteria and increase the content of shortchain fatty acids.