采用高效液相色谱法同时测定醋延胡索中延胡索乙素和延胡索甲素的含量

Simultaneous Determination of Tetrahydropalmatine and Corydaline in Vinegar-steamed Corydalis Rhizoma by HPLC

目的:开发一种高效液相色谱法(HPLC)同时测定醋延胡索中延胡索乙素和延胡索甲素的含量。方法:采用HPLC法,色谱柱选用Kromasil 100-5-C18柱,流动相为甲醇-0.1%磷酸溶液(三乙胺调pH值至6.0)(55∶45),流速:1.0 mL·min^(-1),波长:280 nm下检测,柱温:30℃。结果:在该色谱条件下,两个对照品均达到基线分离,线性关系良好(r>0.9995,n=5);平均加样回收率为96.90%~100.05%,RSD为0.7%~1.9%。结论:该检测方法简便、灵敏、稳定、可靠,可作为醋延胡索的质量控制方法之一。

Objective:To develop a high-performance liquid chromatography(HPLC)method for simultaneous determination of tetrahydropalmatine and tetrahydropalmatine in vinegar yanhusuo.Method:HPLC was used,with Kromasil 100-5-C18 column as the chromatographic column.The mobile phase was methanol 0.1%phosphoric acid solution(adjusted to pH 6.0 with triethylamine)(55:45),with a flow rate of 1.0 mL·min^(-1) and detection at a wavelength of 280 nm.The column temperature was 30℃.Result:Under the chromatographic conditions,both control samples achieved baseline separation with a good linear relationship(r>0.9995,n=5);the average recovery rate was 96.90%~100.05%,and the RSD was 0.7%~1.9%.Conclusion:This detection method is simple,sensitive,stable,and reliable,and can be used as one of the quality control methods for vinegar yanhusuo.