毛建草愈伤组织诱导及植株再生

Callus Induction and Plant Regeneration of Dracocephalum rupestre

毛建草(Dracocephalumrupestre)是一种重要的药用植物。然而,其叶片外植体再生系统尚未建立。以毛建草大田叶片和组培苗叶片为外植体,探讨植物生长调节剂对愈伤组织诱导和分化、不定芽增殖及生根的影响,建立了叶片离体再生体系。结果表明,大田叶片愈伤组织诱导的最适培养基为MS+1.0 mg·L^(-1)6-BA+0.1 mg·L^(-1)2,4-D+1.0 mg·L^(-1)IAA,诱导率达84.51%,不定芽分化最佳培养基为MS+3.0 mg·L^(-1)6-BA+0.5 mg·L^(-1)TDZ+0.5 mg·L^(-1)IAA,分化率为66.37%;组培苗叶片愈伤组织诱导的最适培养基为MS+2.0mg·L^(-1)6-BA+0.1mg·L^(-1)2,4-D+0.5mg·L^(-1)IAA,诱导率达86.73%,不定芽分化最佳培养基为MS+2.0mg·L^(-1)6-BA+2.0mg·L^(-1)TDZ+0.05mg·L^(-1)IAA,分化率为53.48%。不定芽增殖适宜培养基为MS+2.0mg·L^(-1)6-BA+0.05mg·L^(-1)NAA,增殖率为83.57%,最适生根培养基为1/2MS+0.1mg·L^(-1)NAA+0.1mg·L^(-1)IBA,生根率为86.97%;在草炭:蛭石=1:1(v/v)的混合基质中组培苗长势最好。该研究建立了毛建草叶片离体培养再生体系,为毛建草种质资源保存和种苗快繁提供了技术支持。

Dracocephalum rupestre is an important medicinal plant.However,its leaf explant regeneration system has yet to be established.Here we investigated the impact of plant growth regulators on callus induction,differentiation,ad-ventitious bud proliferation,and rooting using field planted leaves and tissue-cultured seedling leaves of D.rupestre as explants.We found that the optimal medium for inducing callus from field planted leaves was MS+1.0 mg·L^(-1)6-BA+0.1 mg·L^(-1)2,4-D+1.0 mg·L^(-1) IAA,achieving an induction rate of 84.51%.For adventitious bud differentiation,the pre-ferred medium comprised MS+3.0 mg·L^(-1)6-BA+0.5 mg·L^(-1) TDZ+0.5 mg·L^(-1) IAA,resulting in a differentiation rate of 66.37%.Similarly,for inducing callus from tissue-cultured seedling leaves,the optimal medium included MS+2.0 mg·L^(-1)6-BA+0.1 mg·L^(-1)2,4-D+0.5 mg·L^(-1) IAA,with an induction rate of 86.73%.The medium conductive to adventitious bud differentiation consisted of MS+2.0 mg·L^(-1)6-BA+2.0 mg·L^(-1) TDZ+0.05 mg·L^(-1) IAA,yielding a differentiation rate of 53.48%.Furthermore,the appropriate medium for adventitious bud proliferation was MS+2.0 mg·L^(-1)6-BA+0.05 mg·L^(-1) NAA,achieving a proliferation rate of 83.57%.The rooting medium was 1/2MS+0.1 mg·L^(-1) NAA+0.1 mg·L^(-1) IBA,resulting in a rooting rate of 86.97%.Tissue-cultured seedlings exhibited optimal growth in a mixed substrate of peat and vermicu-lite at a ratio of 1:1(v/v).This study successfully established a leaf explant regeneration system for D.rupestre,providing valuable technical support for the conservation and rapid propagation of its germplasm resources.