大叶藤黄种子的愈伤组织及丛生芽的诱导

Induction of Callus and Adventitious Buds of Garcinia xanthochymus Seeds

大叶藤黄Garcinia xanthochymus种子具有全能萌发的特点,但其生理基础仍然不明确。为了研究其再生生长特性,分别以大叶藤黄成熟和未成熟种子的切片为外植体,MS为基本培养基,探讨添加外源NAA、TDZ、IAA、6-BA、2,4-D等生长调节物质诱导愈伤组织和不定芽的效果。结果表明:(1)愈伤组织的发生主要取决于种子是否成熟。取自成熟种子的外植体,即使不添加外源生长调节物质,诱导率也超过80%;而取自未成熟种子的外植体,仅TDZ(0.25~3 mg·L^(–1))和6-BA(1.5~2 mg·L^(–1))有效,且发生率低于40%。(2)仅6-BA可稳定诱导生成不定芽,最佳方案为取成熟种子外植体在含5.5 mg·L^(–1)6-BA的MS培养基上培养诱导,其发生率可达73%,外植体的平均不定芽发生数量为5.07个。该研究结果进一步证明大叶藤黄种子具有强大的再生生长能力,同时所研究的幼苗再生方案也为大叶藤黄无性繁育提供了可靠途径。

Garcinia xanthochymus seeds exhibit the characteristic of totipotent germination,yet therein physiological basis remains unclear.To investigate the regenerative growth characteristics,explants from mature and immature seeds of G.xanthochymus were utilized to examine the impacts of growth regulators(NAA,TDZ,IAA,6-BA,and 2,4-D)on callus induction and adventitious bud formation,with MS as basic media.The results indicated that:(1)Callus formation primarily depended on the maturity of the seeds.Explants derived from mature seeds exhibited induction rates exceeding 80%even without exogenous growth regulators,while explants sourced from immature seeds only responded to TDZ(0.25–3 mg·L^(–1))and 6-BA(1.5–2 mg·L^(–1))with a callus induction rate below 40%.(2)Only 6-BA induced adventitious buds steadily in this study,with the optimal approach being the cultivation of explants from mature seeds on MS medium supplemented with 5.5 mg·L^(–1)6-BA,resulting in a 73%incidence rate and an average of 5.07 adventitious shoots each explant.These findings further validated the strong regenerative growth potential of G.xanthochymus seeds,and the investigated plantlet regeneration protocol provided a reliable method for asexual propagation in G.xanthochymus.