黄花型扁蓿豆叶绿体基因组及其系统发育分析

Analysis of Chloroplast Genome and Phylogeny of Yellow Flowered Type Medicago ruthenica

本研究对黄花型扁蓿豆的叶绿体基因组进行序列特征、密码子偏好性分析,并结合系统发育树及共线性分析探究黄花型扁蓿豆与其他近缘种的亲缘关系,以明确其物种分类。结果显示:黄花型扁蓿豆叶绿体基因组全长127572 bp,GC含量34.19%;密码子偏好性分析表明,其叶绿体基因在编码过程中对A/U结尾密码子的使用具有偏好性;系统发育树及共线性分析表明,野生黄花型扁蓿豆叶绿体基因组与青藏扁蓿豆具有更高的相似性。

In this study,the sequence characteristics and codon bias of the chloroplast genome of Yellow flowered type Medicago ruthenica was analyzed,and the phylogenetic tree and collinearity analysis were used to explore the relationship between Yellow flowered type M.ruthenica and other related species,to clarify its species classification.The results showed that the chloroplast genome of Yellow flow‑ered type M.ruthenica was 127572 bp in length and 34.19%in GC content,and the codon bias analysis showed that the coding process of the chloroplast genes used A/U codons preferentially in coding process.Phylogenetic tree and collinearity analysis showed that the chloroplast genome of Yellow flowered type M.ruthenica was more similar to that of Medicago archiducis-nicolai.