甜菜C_(2)H_(2)型锌指蛋白转录因子家族全基因组鉴定及镉胁迫下的表达分析

Genome-Wide Identification and Expression Analysis of C_(2)H_(2) Zinc-Finger Protein Transcription Factor Familyin Sugar Beet under CadmiumStress

甜菜作为一类新兴的能源作物,在重金属污染土壤的修复中具有潜在的优势。探究镉胁迫下甜菜的应答机制,对于培育耐重金属胁迫的甜菜新种质以及生态修复具有重大意义。锌指蛋白转录因子(zinc-finger proteintranscription factor)是植物中广泛存在的基因家族,全方面参与植物的生长发育,并响应生物和非生物胁迫。本研究通过全基因组鉴定以及理化性质、基因结构、保守基序、染色体定位和顺式作用元件分析发现,甜菜体内共有32个C_(2)H_(2)型锌指蛋白转录因子家族成员,可分为3个亚族,各成员具有数量不等的锌指结构域且不同亚族成员的基因结构及序列特征存在较大差异。通过对镉胁迫下的甜菜C_(2)H_(2)型锌指蛋白转录因子的表达和差异表达基因的调控网络分析发现,BVRB_6g137660、BVRB_8g189640、BVRB_1g000840和BVRB_007840在镉胁迫下表达量均不同程度上调,且可能调控了镉胁迫下甜菜金属耐受蛋白及ABC转运蛋白等重要基因的表达,推测这些转录因子在提高甜菜镉耐受性方面起到了关键作用。

As an emerging energy crop,sugar beet has potential advantages in the remediation of heavy metal polluted soil.Exploring the response mechanism of sugar beet under cadmium stress is of great significance for breeding new germplasm resistant to heavy metal stress and ecological restoration.Zinc-finger proteintranscription factor is a widely existing gene family in plants,which takes part in plant growth and development in various aspects and responds to biological and abiotic stresses.Through genome-wide identificationandanalysis of physicochemical properties,gene structure,conserved motifs,chromosome location and cis-element,we found that there were 32 members of the C_(2)H_(2) zinc-finger protein transcription factor family in sugar beet,which could be divided into three subfamilies.Each member had different zinc-finger domains in different numbers,and the gene structure and sequence characteristics of different subfamilies were significantly different.By analyzing the expression of C_(2)H_(2) zinc-finger protein transcription factors and the regulatory networkof differentially expressed genes in sugar beet under cadmium stress,we found that the expression levels of BVRB_6g137660,BVRB_8g189640,BVRB_1g000840 and BVRB_007840 were up-regulated to varyingdegrees under cadmium stress.Speculating that these transcription factors play a key role in improvingthecadmium tolerance of sugar beet.