晋东南小麦品种(系)部分抗病基因的KASP标记检测

Identification of Disease Resistance-Related Genes of WheatCultivars(Lines)in Southeastern Shanxi by KASPAssays

为明确晋东南地区小麦品种(系)中抗病基因分布,利用7套包括条锈病、叶锈病、白粉病的抗性基因相关KASP标记对晋东南地区47个品种(系)进行基因检测。结果显示,在47份材料中,有40份含有抗叶锈病基因Lr14a,占参试材料的85.1%;未检测到抗叶锈病基因Lr34;有4份检测到抗叶锈病基因Lr46,占比8.5%;有12份含有抗叶锈病基因Lr68,占比25.5%;未检测到条锈病抗性基因Yr15;只有1份材料含有白粉病抗性基因Pm21。在检测的6个抗病基因中,只有1份材料(16-7885)含有3个基因(Lr14a、Lr46、Lr68),且全部为叶锈病抗性基因;14份材料含有2个抗病基因,其中11份材料(长4853、长5553、12-9499、16-7381、16-7527、17-9627、17-6665、17-7103、17-6925、17-6755、17-9467)含有2个叶锈病抗性基因(Lr14a和Lr68);2份材料(长6359、长6990)含有(2个叶锈病抗性基因Lr14a和Lr46);1份材料(17-6891)含有白粉病抗性基因Pm21和叶锈病抗性基因Lr46。

In order to clarify the distribution of resistance genes in wheat varieties(lines)in southeasternShanxi,seven pairs of KASP markers relating stripe rust,leaf rust and powdery mildew resistance genes were usedforgene detection of 47 varieties(lines)in southeastern Shanxi.The results showed that in all 47 tested materials,40materials contained the leaf rust resistance gene Lr14a,accounting for 85.1%of the total materials;Leaf rust resistance gene Lr34 was not detected;The leaf rust resistance gene Lr46 was detected in four materials,accounting for 8.5%of the total materials;There were 12 materials containing leaf rust resistance geneLr68,accounting for 25.5%of the total materials;No stripe rust resistance gene Yr15 was detected;Only one material contained powdery mildew resistance gene Pm21.Among the six resistance genes detected,only one material(16-7885)contained three genes(Lr14a,Lr46,Lr68),and all of them were leaf rust resistance genes.Therewere14 materials containing two resistance genes,in which 11 materials(Chang 4853,Chang 5553,12-9499,16-7381,16-7527,17-9627,17-6665,17-7103,17-6925,17-6755,17-9467)contained two leaf rust resistance genes(Lr14a and Lr68),two materials(Chang 6359,Chang 6990)contained two leaf rust resistance genes(Lr14aandLr46),one material(17-6891)contained powdery mildew resistance gene Pm21 and leaf rust resistance gene Lr46.