CMTM4和PD-L1蛋白在胰腺癌组织中的表达及其与临床病理特征的关系

Expression of CMTM4 and PD-L1 Proteins in Pancreatic Cancer and Their Relationship with Clinicopathological Features

目的:探讨CMTM4和PD-L1蛋白在胰腺癌组织中的表达状况,并分析二者与临床病理特征的关系。方法:收集2016.1月-2020.1我院及内蒙古医科大学附属医院胰腺癌手术标本40例作为实验组,并同时选取癌旁组织作为对照组,采用免疫组化Envision二步法染色检测胰腺癌组织中CMTM4和PD-L1蛋白表达,统计病理特征,CMTM4和PD-L1蛋白在癌组织、癌旁组织中的表达,以及二者与临床病理特征关系。结果:CMTM4主要分布在肿瘤细胞膜和细胞质中,均呈棕褐色或棕黄色颗粒;PD-L1主要分布在肿瘤间质免疫细胞、细胞膜和细胞质中,呈棕褐色或淡黄色。胰腺癌组织CMTM4蛋白、PD-L1蛋白水平明显高于癌旁组织(P<0.05)。与癌旁组织相比,胰腺癌组织CMTM4蛋白、PD-L1蛋白高表达高,CMTM4蛋白、PD-L1蛋白低表达均明显低于癌旁组织(P<0.05)。CMTM4蛋白高表达、PD-L1蛋白高表达的神经侵犯、淋巴结转移的发病率均高于CMTM4蛋白低表达、PD-L1蛋白低表达(P<0.05);但性别、年龄、肿瘤部位、肿瘤直径、组织分化程度、TNM分期,CMTM4蛋白高表达、PD-L1蛋白高表达与CMTM4蛋白低表达、PD-L1蛋白低表达比较(P>0.05)。CMTM4蛋白、PD-L1蛋白表达与胰腺癌的组织分化程度、TNM分期无关(P>0.05)。结论:CMTM4和PD-L1蛋白在胰腺癌组织中呈高表达,其与神经侵犯、淋巴结转移相关。

Objective:To explore the expression status of CMTM 4 and PD-L1 protein in pancreatic cancer tissues and analyzed the relationship between the clinician and clinicopathological features.Methods:From January 2016 to 2020.1,our Hospital of Inner Mongolia Medical University collected 40 cases as the experimental group,and selected adjacent tissues as the control group,to detect the expression of CMTM 4 and PD-L1 protein by immunohistochemistry Envision,the expression of CMTM 4 and PD-L1 protein in cancer tissues and adjacent tissues,and the relationship between the two and clinical pathological features.Results:CMTM 4 is mainly distributed in tumor cell membrane and cytoplasm,both brown or brown particles;PD-L1 is mainly distributed in tumor stromal immune cells,cell membrane and cytoplasm,brown or light yellow.The levels of CMTM 4 and PD-L1 in pancreatic cancer were higher than those in adjacent cancer(P<0.05).Compared with adjacent tissues,pancreatic cancer tissues had high expression of CMTM 4 protein and PD-L1 protein,and low expression of CMTM 4 protein and PD-L1 protein was lower than adjacent tissues(P<0.05).The incidence of nerve invasion with high PD-L1 expression and lymph node metastasis was higher than that of CMTM 4 and PD-L1 expression(P<0.05);however,gender,age,tumor site,tumor diameter,tissue differentiation,TNM stage,high CMTM 4 expression and high PD-L1 protein expression and low CMTM 4 protein and low PD-L1 expression(P>0.05).CMTM 4 protein and PD-L1 protein expression were not correlated with the degree of tissue differentiation or TNM stage of pancreatic cancer(P>0.05).Conclusion:CMTM4 and PD-L1 proteins are highly expressed in pancreatic cancer tissues,which are related to nerve invasion and lymph node metastasis.