复发性流产患者血清IL-33/ST2轴水平的表达及其预测价值分析

Expression of Serum IL-33/ST2 Axis Levels in Patients with Recurrent Spontaneous Abortion and Analysis of Their Predictive Value

目的:探讨复发性流产(RSA)患者血清白细胞介素-33(IL-33)/生长刺激表达基因2(ST2)轴水平的表达及其预测价值。方法:选取2021年1月~2023年1月苏州大学附属第二医院就诊的80例RSA患者为RSA组,另选取同期80名无异常妊娠史的孕妇为对照组。通过酶联免疫吸附法检测血清IL-33、可溶性ST2(sST2)水平。采用Pearson相关性分析RSA患者血清IL-33与sST2水平的相关性,采用多因素Logistic回归分析影响RSA的因素,采用受试者工作特征(ROC)曲线分析血清IL-33、sST2水平对RSA的预测价值。结果:与对照组比较,RSA组血清IL-33、sST2水平升高(P<0.05)。Pearson相关性分析显示,RSA患者血清IL-33与sST2水平呈正相关(r=0.746,P<0.001)。多因素Logistic回归分析显示,生殖道感染、血清IL-33、sST2水平升高为RSA发生的独立危险因素(P<0.05)。ROC曲线分析显示,血清IL-33、sST2水平联合预测RSA的AUC为0.888,大于血清IL-33、sST2水平单独预测的0.794、0.840。结论:RSA患者血清IL-33、sST2水平升高是RSA发生的独立危险因素,IL-33/ST2轴水平异常表达参与RAS发生过程。血清IL-33、sST2水平联合检测对RSA具有较高的预测价值。

Objective: To investigate the expression of serum interleukin-33(IL-33)/suppression of tumorigenicity 2(ST2) axis levels and its predictive value in patients with recurrent spontaneous abortion(RSA). Methods: 80 patients with RSA treated in The Second Affiliated Hospital of Soochow University from January 2021 to January 2023 were selected as the RSA group, and another 80 pregnant women with no history of abnormal pregnancy who underwent abortion during the same period were selected as the control group.Serum IL-33 and soluble ST2(s ST2) levels were measured by enzyme-linked immunosorbent assay. Pearson correlation was used to analyze the correlation between serum IL-33 and s ST2 levels in patients with RSA, multivariate Logistic regression was used to analyze the factors affecting RSA, and the predictive value of serum IL-33 and s ST2 levels for RSA was analyzed by receiver operating characteristic(ROC) curve. Results: Compared with the control group, the serum IL-33 and s ST2 levels in the RSA group increased(P<0.05). Pearson correlation analysis showed a positive correlation between serum IL-33 and s ST2 levels in patients with RSA(r=0.746, P<0.001). Multivariate Logistic regression analysis showed that reproductive tract infection, elevated serum IL-33 and s ST2 levels were independent risk factors for RSA(P<0.05). ROC curve analysis showed that the AUC of RSA predicted by the combination of serum IL-33 and s ST2 levels was 0.888, which was higher than the 0.794 and 0.840 predicted by serum IL-33 and s ST2 levels alone. Conclusion: Elevated serum IL-33 and s ST2 levels in RSA patients are independent risk factors for the occurrence of RSA, and abnormal expression of IL-33/ST2axis levels is involved in the process of RAS. The combined detection of serum IL-33 and s ST2 levels has high predictive value for RSA.