多种中药注射液浸泡对自体颗粒脂肪移植存活率的观察

Observation on the survival rate of autologous granule fat grafting by immersion in a variety of traditional Chinese medicine injections

目的:观察局部使用中药注射液(丹参、黄芪、红花)对自体脂肪移植后存活率的影响。方法:将15只新西兰大白兔模型,均采集双侧腹股沟脂肪组织,制备脂肪颗粒并移植至双侧兔耳,随机分为5组,除一组不予干预,其余分别予氯化钠注射液、黄芪注射液、丹参注射液、红花注射液浸泡脂肪颗粒,分别在移植术后第2周、第4周、第12周进行取材,通过HE染色和CD31免疫组化对移植脂肪进行观察,综合评估移植体存活情况。结果:自体脂肪移植术后第12周,中药实验组在细胞形态、新生毛细血管数量等方面均明显优于对照组。结论:在移植前用中药注射液局部浸泡颗粒脂肪,对移植后提高脂肪存活率有积极影响,且不同中药注射液具有不同的调节作用,在改善局部血液微循环,促进新生血管方面,黄芪注射液效果最为显著;在细胞形态的改善方面,丹参注射效果更佳。

Objective:Exploring the effect of local use of traditional Chinese medicine injections(Salvia miltiorrhiza,Astragalus,Safflower)on the survival rate after autologous fat transplantation.Methods:Fifteen New Zealand white rabbit models were selected and randomly divided into 5groups.Bilateral inguinal adipose tissue was collected and fat particles were prepared.Except for one group that did not intervene,fat particles were soaked in sodium chloride injection,astragalus injection,salvia miltiorrhiza injection,and safflower injection.Samples were collected at 2weeks,4weeks,and 12weeks after transplantation,and HE staining of transplanted fat and CD31expression of endothelial cells were observed to comprehensively evaluate the transplantation survival rate.Results:At the 12th week after autologous fat transplantation,the experimental group was significantly better than the pure fat group and sodium chloride group in terms of cell morphology,number of new capillaries,and cell activity.Conclusion:Local soaking with traditional Chinese medicine injection before granular fat transplantation has a positive effect on improving fat survival rate after transplantation,and different traditional Chinese medicine injections have different regulatory effects.Astragalus injection has the most prominent effect in improving local blood microcirculation and promoting neovascularization.The injection of salvia miltiorrhiza had better effect on the improvement of cell morphology.