ADAM12、MUC3A和MUC17在胃癌中的表达及生存预后分析

Expression of ADAM12,MUC3A and MUC17 in gastric cancer and analysis of survival prognosis

目的探讨去整合素金属蛋白酶12(a disintergin and metalloprotease 12,ADAM12)、黏蛋白3A(mucin 3A,MUC3A)和黏蛋白17(mucin 17,MUC17)基因在胃癌中的表达及生存预后关系。方法收集癌症基因组图谱(The Cancer Genome Atlas,TCGA)、基因表达谱交互分析2(Gene Expression Profiling Interactive Analysis 2,GEPIA2)数据库信息,采用R 4.2.2和SPSS软件分析ADAM12、MUC3A和MUC17基因在胃癌中的差异性表达、生存预后关系、泛癌表达,以及不同临床病理特征与基因表达量之间的相关性。通过人类蛋白质图谱(Human Protein Atlas,HPA)数据库分析三种基因在正常组织中的表达水平。利用基因本体论(Gene Ontology,GO)和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)功能富集分析评估三种基因在胃癌中的潜在功能。采用实时荧光定量聚合酶链反应(quantitative real-time polymerase chain reaction,q-PCR)进一步验证肿瘤组织与癌旁组织中ADAM12、MUC3A和MUC17的表达情况。结果生物信息学及q-PCR结果显示,ADAM12、MUC3A和MUC17在正常组织中表达量较低,在胃癌组织中表达量均被上调(P<0.05),且ADAM12在胃癌中伴随着不良预后(P<0.05)。三种基因在肿瘤相关通路富集,与胃癌的发生发展有关。结论ADAM12、MUC3A和MUC17在胃癌中高表达,其中ADAM12是胃癌预后的影响因素。三种基因参与肿瘤的进展,均有可能成为胃癌潜在的治疗靶点。

Objective To investigate the expression of a disintergin and metalloprotease 12(ADAM12),mucin 3A(MUC3A)and mucin 17(MUC17)in gastric cancer and the relationship between them and survival prognosis.Methods Information from The Cancer Genome Atlas(TCGA)and Gene Expression Profiling Interactive Analysis 2(GEPIA2)databases was collected,and R 4.2.2 as well as SPSS software were used to analyse the differential expression,survival-prognosis relationship,pan-cancer expression,and correlation between different clinicopathological features and expressions of ADAM12,MUC3A and MUC17 genes in gastric cancer.The expression levels of the three genes in normal tissues were analysed by Human Protein Atlas(HPA)database.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses were used to evaluate the potential functions of the three genes.The expression of ADAM12,MUC3A and MUC17 in tumour tissues and paracancerous tissues was further verified using quantitative real-time polymerase chain reaction(q-PCR).Results Bioinformatics analysis and q-PCR results showed that the expression of ADAM12,MUC3A and MUC17 were low in normal tissues and all upregulated in gastric cancer(P<0.05),and ADAM12 was accompanied by a poor prognosis in gastric cancer(P<0.05).The three genes were enriched in tumor-related pathways and associated with the development of gastric cancer.Conclusion ADAM12,MUC3A and MUC17 show high expression in gastric cancer,of which ADAM12 is an influencing factor in the prognosis of gastric cancer.All three genes are associated with the progression of tumors and may be potential therapeutic targets for gastric cancer.